Quantitative analysis of alpha and beta adrenergic receptor densities in the lower urinary tract of the dog and the rabbit.
Quantitative analysis of alpha and beta adrenergic receptor densities in the lower urinary tract of the dog and the rabbit.
复制标题
狗和兔子下尿路α和β肾上腺素能受体密度的定量分析。
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
A. Wein
中科院分区：
文献类型：
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作者：
R. Levin;A. Wein
使用特异性放射性配体受体结合测定，我们首次定量了狗的下输尿管、膀胱体、后膀胱基底部和近端尿道的肌肉组织以及兔子的膀胱基底部和身体中的α和β肾上腺素能受体。对于β受体测定，使用3H-二氢阿普洛尔作为配体，对于α受体测定，使用3H-二氢麦角隐亭。在狗身上，膀胱体中的β受体密度明显高于输尿管或后膀胱基底部。尿道具有中等密度。膀胱基部和近端尿道的α受体密度明显高于输尿管或膀胱穹顶。对于所有研究的组织，DHA β 受体的解离常数约为 9 nM，DHE α 受体的解离常数约为 7 nM。在兔膀胱中观察到α-和β肾上腺素能受体的类似分布。
Using specific radioligand receptor binding assays, we have, for the first time, quantitated both alpha- and beta-adrenergic receptors in the musculature of the lower ureter, bladder body, posterior bladder base, and proximal urethra of the dog, and in the bladder base and body of the rabbit. For the beta receptor assay 3H-dihydroalprenolol was used as the ligand, and, for the alpha receptor assay, 3H-dihydroergocryptine was used. In the dog, there was a significantly greater density of beta receptors in the bladder body than in either the ureter or the posterior bladder base. The urethra had an intermediate density. The bladder base and the proximal urethra had a significantly greater density of alpha receptors than either the ureter or the bladder dome. The dissociation constant of beta receptors for DHA was approximately 9 nM for all tissues studied, and that of alpha receptors for DHE approximately 7 nM. A similar distribution of alpha-and beta adrenergic receptors was seen in the rabbit bladder.