Electrochemical cotinine sensing with a molecularly imprinted polymer on a graphene-platinum nanoparticle modified carbon electrode towards cigarette smoke exposure monitoring
Electrochemical cotinine sensing with a molecularly imprinted polymer on a graphene-platinum nanoparticle modified carbon electrode towards cigarette smoke exposure monitoring
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DOI：
10.1016/j.snb.2019.02.032
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发表时间：
2019-05
期刊：
影响因子：
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通讯作者：
Kshama Parate;C. Karunakaran;J. Claussen
中科院分区：
文献类型：
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作者：
Kshama Parate;C. Karunakaran;J. Claussen
可替宁是尼古丁的代谢物，由于其在烟草消费后在人体体液中持续数天（约4 -5天）的能力，已显示出作为检测烟草使用和烟雾暴露的生物标志物的前景。然而，目前的可替宁检测策略主要包括艰苦的实验室传感方法或定性现场生物传感装置。在此，我们报告了一种电化学可替宁传感器的基础上选择性的分子印迹聚合物（MIP）电沉积在丝网印刷碳电极（SPCE）与石墨烯片和铂纳米粒子（PtNPs）改性。PtNP-石墨烯修饰的SPCE在铁氰化物循环伏安法中表现出4倍的电化学灵敏度（10 μA-40 μA）。因此，这种开发的与MIP功能化的生物传感器能够在宽的传感范围（1-100 nM）和低检测限（0.33 nM）的范围内选择性地检测加标唾液样品中的可替宁。该传感范围涵盖了非吸烟者和吸烟者唾液中通常存在的可替宁浓度水平（约10 -75 nM）。此外，传感能够在12分钟内获得可替宁测量值，尼古丁和麦斯明-可替宁化学类似物（通常在烟草产品中发现）的干扰最小。因此，所开发的生物传感器非常适合在诸如护理点设施的领域中使用。
Cotinine, a metabolite of nicotine, has shown promise as a biomarker for the detection of tobacco use and smoke exposure due its ability to persist in human bodily fluids for days (ca.4–5 days) after tobacco consumption. However, current cotinine detection strategies primarily include arduous laboratory sensing methods or qualitative in-field biosensing devices. Herein, we report an electrochemical cotinine sensor based on a selective molecularly-imprinted polymer (MIP) electrodeposited on a screen-printed carbon electrode (SPCE) modified with graphene flakes and platinum nanoparticles (PtNPs). The PtNP-graphene modified SPCE exhibited a 4-fold increase in electrochemical sensitivity (10 μA–40 μA) during ferricyanide cyclic voltammetry. This developed biosensor functionalized with the MIP was consequently capable of selective sensing of cotinine in spiked saliva samples across a wide sensing range (1–100 nM) and low detection limit of (0.33 nM). This sensing range covers cotinine concentration levels that are typically found in saliva for non-smokers and smokers (ca.10–75 nM). Moreover, the sensing is capable of acquiring a cotinine measurement within 12 min with minimal interference from both nicotine and myosmine– cotinine chemical analogs that are typically found in tobacco products. Hence, the developed biosensor is well-suited for use in the field such as at point-of-care facilities.