Effects of mutating putative two-component systems on biofilm formation by Streptococcus mutans UA159
Effects of mutating putative two-component systems on biofilm formation by Streptococcus mutans UA159
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DOI：
10.1111/j.1574-6968.2001.tb10952.x
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发表时间：
2001-12-18
影响因子：
2.1
通讯作者：
Burne, RA
中科院分区：
文献类型：
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作者：
Bhagwat, SP;Nary, J;Burne, RA
变形链球菌具有至少六对与细菌双组分调节系统同源的开放阅读框。通过插入卡那霉素抗性标记，成功突变了六对中的五对假定的反应调节因子，并评估了基因失活对细胞在体外模型中形成生物膜的能力的影响。与野生型变形链球菌相比，四种系统的反应调节剂的破坏对生物膜形成没有影响，而一种反应调节剂的破坏导致生物膜形成显着减少。 (C) 2001 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
Streptococcus mutans has at least six pairs of open reading frames that are homologous to bacterial two-component regulatory systems. Putative response regulators from five out of six of these pairs were successfully mutated by insertion of a kanamycin resistance marker and the effects of inactivation of the genes on the ability of the cells to form biofilms in an in vitro model were assessed. Disruption of the response regulators of four systems had no effect on biofilm formation, whereas disruption of one response regulator caused a substantial decrease in biofilm formation as compared to the wild-type S. mutans. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.