Environmental and Genetic Factors Regulating Localization of the Plant Plasma Membrane H+-ATPase
Environmental and Genetic Factors Regulating Localization of the Plant Plasma Membrane H+-ATPase
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DOI：
10.1104/pp.17.01126
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发表时间：
2018-01-01
期刊：
影响因子：
7.4
通讯作者：
Sussman, Michael R.
中科院分区：
文献类型：
--
作者：
Haruta, Miyoshi;Tan, Li Xuan;Sussman, Michael R.
P型H+-ATP酶是高等植物质膜上将ATP转化为电化学能的主要转运蛋白。它的产物，质子动力，由电势和pH梯度组成。许多研究表明，这种质子动力不仅驱动营养摄取所需的次级转运蛋白，而且在调节细胞扩张中起直接作用。在这里，我们已经产生了一个转基因拟南芥（拟南芥）植物表达H+-ATP酶异构体2（AHA 2），这是一种荧光蛋白的融合，并检查其细胞定位活细胞显微镜。使用3D成像方法与幼苗生长的各种时间下的各种光强度，我们证明，AHA 2定位在根细胞的质膜需要光。在弱光条件下，除了质膜外，AHA 2还存在于细胞内隔室中。这种定位配置文件是年龄依赖性和特定的细胞类型中发现的分生组织和伸长区之间的过渡区。AHA 2在细胞内隔室中的积累与过渡区附近的H+分泌减少和根生长的抑制是一致的。通过检查AHA 2定位在受体蛋白激酶，FERONIA的敲除突变体，我们发现，AHA 2在过渡区的细胞内积累是依赖于功能FERONIA依赖性抑制反应在根伸长。总的来说，这项研究提供了一个分子基础，了解遗传，环境和发育因素影响根生长通过定位质膜H+-ATP酶。
A P-type H+-ATPase is the primary transporter that converts ATP to electrochemical energy at the plasma membrane of higher plants. Its product, the proton-motive force, is composed of an electrical potential and a pH gradient. Many studies have demonstrated that this proton-motive force not only drives the secondary transporters required for nutrient uptake, but also plays a direct role in regulating cell expansion. Here, we have generated a transgenic Arabidopsis (Arabidopsis thaliana) plant expressing H+-ATPase isoform 2 (AHA2) that is translationally fused with a fluorescent protein and examined its cellular localization by live-cell microscopy. Using a 3D imaging approach with seedlings grown for various times under a variety of light intensities, we demonstrate that AHA2 localization at the plasma membrane of root cells requires light. In dim light conditions, AHA2 is found in intracellular compartments, in addition to the plasma membrane. This localization profile was age-dependent and specific to cell types found in the transition zone located between the meristem and elongation zones. The accumulation of AHA2 in intracellular compartments is consistent with reduced H+ secretion near the transition zone and the suppression of root growth. By examining AHA2 localization in a knockout mutant of a receptor protein kinase, FERONIA, we found that the intracellular accumulation of AHA2 in the transition zone is dependent on a functional FERONIA-dependent inhibitory response in root elongation. Overall, this study provides a molecular underpinning for understanding the genetic, environmental, and developmental factors influencing root growth via localization of the plasma membrane H+-ATPase.