Prognostic value of tumor-associated macrophage count in human bladder cancer
Prognostic value of tumor-associated macrophage count in human bladder cancer
复制标题
DOI：
10.1046/j.1442-2042.2000.00190.x
复制
发表时间：
2000-07-01
影响因子：
2.6
通讯作者：
Nomura, Y
中科院分区：
文献类型：
--
作者：
Hanada, T;Nakagawa, M;Nomura, Y
背景资料：为探讨肿瘤相关巨噬细胞（TAM）计数对膀胱癌患者临床预后的预测价值，采用免疫组化法检测了63例膀胱癌患者的TAM计数和微血管计数（MVC），其中浅表性膀胱癌40例，浸润性膀胱癌23例。为探讨TAM计数与膀胱癌临床结局或预后的关系，比较TAM计数低（< 67）和高（< 67）的膀胱癌患者的膀胱移行细胞率、远处转移率、血管浸润率和5年生存率。（大于或等于67）TAM计数。浸润性膀胱癌的TAM计数（154.22 ± 11.98）显著高于浅表性膀胱癌（49.05 ± 7.76; P < 0.0001）。浸润性膀胱癌的MVC（71.55 ± 10.44）也显著高于表浅性膀胱癌（47.02 ± 5.57; P < 0.05）。TAM计数与MVC呈正相关（r = 0.30; P = 0.02）。CD 68/辣根过氧化物酶单克隆抗体免疫组化染色显示浸润性膀胱癌的浸润细胞多于浅表性膀胱癌。TAM计数高（≥ 67）的患者，其膀胱癌、远处转移和血管浸润的发生率明显高于TAM计数低（< 67）的患者。TAM计数高者5年生存率显著低于TAM计数低者（P < 0.0001）。结论：测定膀胱癌组织中TAM计数对预测膀胱癌患者的临床预后及选择适当的治疗方案有一定的价值。
Background: We determined the tumor-associated macrophage (TAM) count to investigate its importance in predicting clinical outcome or prognosis in patients with bladder cancer.Methods: The TAM count and microvessel count (MVC) were determined immunohistochemically in 63 patients with bladder cancer, including 40 superficial bladder cancers and 23 invasive bladder cancers. To examine the relationship between TAM count and clinical outcome or prognosis in bladder cancer, cystectomy rates, distant metastasis rates, vascular invasion rates and 5 year survival rates were compared between patients with low (< 67) and high (greater than or equal to 67) TAM counts.Results: The TAM count in invasive bladder cancers (154.22 +/- 11.98) was significantly higher than in superficial bladder cancers (49.05 +/- 7.76; P < 0.0001). The MVC in invasive bladder cancers (71.55 +/- 10.44) was also significantly higher than in superficial bladder cancers (47.02 +/- 5.57; P < 0.05). There was a positive correlation between TAM count and MVC (r = 0.30; P = 0.02). Immunohistochemical staining using CD68/horseradish peroxidase monoclonal antibody showed more infiltrating cells in invasive than superficial bladder cancers. Patients with a high TAM count (greater than or equal to 67) showed significantly higher rates of cystectomy, distant metastasis and vascular invasion than those with a lower TAM count (< 67). The 5 year survival rate estimated using the Kaplan- Meier method was significantly lower in patients with a high TAM count than in those with a low TAM count (P < 0.0001).Conclusions: Our results suggest that determination of TAM count in bladder cancer tissues is of value to predict the clinical outcome or prognosis and to select appropriate treatment strategies in patients with bladder cancer.