Repression of slow myosin heavy chain 2 gene expression in fast skeletal muscle fibers by muscarinic acetylcholine receptor and G(alpha)q signaling.
Repression of slow myosin heavy chain 2 gene expression in fast skeletal muscle fibers by muscarinic acetylcholine receptor and G(alpha)q signaling.
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通过毒蕈碱乙酰胆碱受体和 G(α)q 信号传导抑制快骨骼肌纤维中的慢肌球蛋白重链 2 基因表达。
DOI：
10.1083/jcb.200303164
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
DiMario,JosephX
中科院分区：
文献类型：
--
作者：
Jordan,Theresa;Li,Jinyuan;Jiang,Hongbin;DiMario,JosephX
Gene expression in skeletal muscle fibers is regulated by innervation and intrinsic fiber properties. To determine the mechanism of repression ofslow MyHC2expression in innervated fast pectoralis major (PM) fibers, we investigated the function of the muscarinic acetylcholine receptor (mAchR) and Gαq. Both mAchR and Gαq are abundant in medial adductor (MA) and PM fibers, and mAchR and Gαq interact in these fibers. Whereas innervation of PM fibers was insufficient to induceslow MyHC2expression, inhibition of mAchR activity with atropine in innervated PM fibers inducedslow MyHC2expression. Increased Gαq activity repressedslow MyHC2expression to nondetectable levels in innervated MA fibers. Reduced mAchR activity decreased PKC activity in PM fibers, and increased Gαq activity increased PKC activity in PM and MA fibers. Decreased PKC activity in atropine-treated innervated PM fibers correlated withslow MyHC2expression. These data suggest thatslow MyHC2repression in innervated fast PM fibers is mediated by cell signaling involving mAchRs, Gαq, and PKC.
