PREVENTING ERRORS WHEN ESTIMATING SINGLE-CHANNEL PROPERTIES FROM THE ANALYSIS OF CURRENT FLUCTUATIONS
PREVENTING ERRORS WHEN ESTIMATING SINGLE-CHANNEL PROPERTIES FROM THE ANALYSIS OF CURRENT FLUCTUATIONS
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DOI：
10.1016/s0006-3495(93)81196-2
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发表时间：
1993-10-01
影响因子：
3.4
通讯作者：
MAGLEBY, KL
中科院分区：
文献类型：
--
作者：
SILBERBERG, SD;MAGLEBY, KL
离子通道的电导、数量和平均打开时间可以通过膜电流的波动来估计。为了检查与波动分析相关的潜在误差，我们模拟了集合电流并估计了单通道特性。使用非平稳波动分析估计底层单通道的数量(N)和幅度(i)，而使用协方差和频谱分析估计平均打开时间。过度滤波和对过短电流段的分析都会导致对i的低估和对n的高估。将滤波器的低通截止频率设置为大于5倍于有效平均通道打开时间（突发持续时间）的倒数，并对至少为有效平均通道打开时间80倍的电流段进行分析，将误差降低到
The conductance, number, and mean open time of ion channels can be estimated from fluctuations in membrane current. To examine potential errors associated with fluctuation analysis, we simulated ensemble currents and estimated single channel properties. The number (N) and amplitude (i) of the underlying single channels were estimated using nonstationary fluctuation analysis, while mean open time was estimated using covariance and spectral analysis. Both excessive filtering and the analysis of segments of current that were too brief led to underestimates of i and overestimates of N. Setting the low-pass cut-off frequency of the filter to greater than five times the inverse of the effective mean channel open time (burst duration) and analyzing segments of current that were at least 80 times the effective mean channel open time reduced the errors to