Comparative analysis of midgut bacterial communities of Aedes aegypti mosquito strains varying in vector competence to dengue virus
Comparative analysis of midgut bacterial communities of Aedes aegypti mosquito strains varying in vector competence to dengue virus
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DOI：
10.1007/s00436-013-3428-x
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发表时间：
2013-07-01
影响因子：
2
通讯作者：
Shouche, Yogesh S.
中科院分区：
文献类型：
--
作者：
Charan, Shakti S.;Pawar, Kiran D.;Shouche, Yogesh S.
登革热病毒（DENV）的主要媒介埃及伊蚊（Aedes aegypti）中肠细菌群落的差异可能影响其对DENV感染的易感性。作为解决这一假设的第一步，对对DENV具有不同遗传易感性的蚊子菌株的中肠细菌群落进行了比较分析。采用16S rRNA基因文库和实时PCR方法对MOYO、MOYO- r和MOYO- s 3株埃及伊蚊中肠细菌群落组成和丰度进行了分析。虽然假单胞菌相关克隆在所有文库中都占主导地位，但在三种菌株的中肠细菌群落中发现了一些有趣且可能显著的差异。Pedobacter sp.-和Janthinobacterium sp.相关的种型仅在MOYO-R菌株文库中检测到，而Bacillus sp.仅在MOYO-S菌株中检测到。在MOYO- r和MOYO菌株文库中均发现Rahnella sp.，但在MOYO- s菌株文库中未发现。16S rRNA基因文库和real-time PCR均证实仅在MOYO-R菌株中存在Pedobacter sp.。此外，基于实时pcr的16S rRNA基因拷贝数定量显示，MOYO- r菌株蚊子中部肠道细菌丰度至少比MOYO- s和MOYO菌株蚊子高10-100倍。我们的研究发现了一些可能影响登革热病毒传染性的具有特征性生理特性的细菌。该分析代表了第一份比较埃及伊蚊对登革热病毒的耐受性和易感性的中肠细菌群落的报告，并将指导未来努力解决埃及伊蚊中肠细菌在确定登革热病毒媒介能力方面的潜在相互作用。
Differences in midgut bacterial communities of Aedes aegypti, the primary mosquito vector of dengue viruses (DENV), might influence the susceptibility of these mosquitoes to infection by DENV. As a first step toward addressing this hypothesis, comparative analysis of bacterial communities from midguts of mosquito strains with differential genetic susceptibility to DENV was performed. 16S rRNA gene libraries and real-time PCR approaches were used to characterize midgut bacterial community composition and abundance in three Aedes aegypti strains: MOYO, MOYO-R, and MOYO-S. Although Pseudomonas spp.-related clones were predominant across all libraries, some interesting and potentially significant differences were found in midgut bacterial communities among the three strains. Pedobacter sp.- and Janthinobacterium sp.-related phylotypes were identified only in the MOYO-R strain libraries, while Bacillus sp. was detected only in the MOYO-S strain. Rahnella sp. was found in MOYO-R and MOYO strains libraries but was absent in MOYO-S libraries. Both 16S rRNA gene library and real-time PCR approaches confirmed the presence of Pedobacter sp. only in the MOYO-R strain. Further, real-time PCR-based quantification of 16S rRNA gene copies showed bacterial abundance in midguts of the MOYO-R strain mosquitoes to be at least 10-100-folds higher than in the MOYO-S and MOYO strain mosquitoes. Our study identified some putative bacteria with characteristic physiological properties that could affect the infectivity of dengue virus. This analysis represents the first report of comparisons of midgut bacterial communities with respect to refractoriness and susceptibility of Aedes aegypti mosquitoes to DENV and will guide future efforts to address the potential interactive role of midgut bacteria of Aedes aegypti mosquitoes in determining vectorial capacity for DENV.