RAPID IDENTIFICATION OF SOME LEPTOSPIRA ISOLATES FROM CATTLE BY RANDOM AMPLIFIED POLYMORPHIC DNA-FINGERPRINTING
RAPID IDENTIFICATION OF SOME LEPTOSPIRA ISOLATES FROM CATTLE BY RANDOM AMPLIFIED POLYMORPHIC DNA-FINGERPRINTING
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DOI：
10.1128/jcm.31.11.2927-2932.1993
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发表时间：
1993-11-01
影响因子：
9.4
通讯作者：
GRAHAM, GC
中科院分区：
文献类型：
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作者：
CORNEY, BG;COLLEY, J;GRAHAM, GC
比较了随机扩增多态性DNA（RAPD）指纹图谱与交叉吸收凝集和限制性内切酶分析对牛钩端螺旋体的分型效果。使用RAPD指纹图谱，我们研究了一些钩端螺旋体血清型，即，hardjo基因型牛和prajitno，pomona，balcanica，tarassovi，swajizak，kremaidae，australis和zanoni，这是可能从澳大利亚牛分离。每个血清型和基因型有一个独特的RAPD图谱。26株钩端螺旋体经RAPD指纹图谱鉴定，23株为Hardjo基因型牛A亚型，2株为Zanoni型，1株为Pomona型，并经交叉吸收凝集试验和限制性内切酶分析确定了它们的分型。
We compared random amplified polymorphic DNA (RAPD) fingerprinting with cross-absorption agglutination and restriction enzyme analysis for typing bovine leptospires. Using RAPD fingerprinting, we examined a number of Leptospira serovars, namely, hardjo genotypes bovis and prajitno, pomona, balcanica, tarassovi, swajizak, kremastos, australis, and zanoni, which are likely to be isolated from Australian cattle. Each serovar and genotype had a unique RAPD profile. Of 26 field isolates of Leptospira, 23 were identified as hardjo genotype bovis subtype A, 2 were identified as zanoni, and 1 was identified as pomona by RAPD fingerprinting, and their types were confirmed by cross-absorption agglutination and restriction enzyme analysis.