Multiplexing potential of NIR resonant and non-resonant Raman reporters for bio-imaging applications.
Multiplexing potential of NIR resonant and non-resonant Raman reporters for bio-imaging applications.
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DOI：
10.1039/d3an01298k
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发表时间：
2023-10
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影响因子：
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通讯作者：
O. Eremina;Sarah Schaefer;Alexander T Czaja;Samer Awad;Matthew A Lim;Cristina Zavaleta
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作者：
O. Eremina;Sarah Schaefer;Alexander T Czaja;Samer Awad;Matthew A Lim;Cristina Zavaleta
多路成像技术允许同时对多个分子特征进行检测，对于解决生物医学领域的众多挑战至关重要。光学上独特的表面增强拉曼散射（SERS）纳米颗粒（NPs）具有在单次采集中实现高复用成像的潜力，该成像是非破坏性的，定量的，且易于执行。当使用激光激发在785 nm时，允许来自生物组织的较低背景，近红外（NIR）染料可以用作拉曼报告，由于共振效应提供高拉曼信号强度。这类显像剂被称为表面增强共振拉曼散射（SERRS） NPs。研究人员在其用于生物医学应用的纳米颗粒结构中主要使用两类拉曼报告器：nir共振和非共振拉曼报告器。在此，我们研究了五种非共振SERS: BPE， 44DP， PTT， PODT和BMMBP，以及五种近红外共振SERRS NP香料与七甲基花菁染料：DTTC， IR-770, IR-780， IR-792和IR-797的多路复用潜力，这些香料已广泛用于生物医学成像应用。虽然由于其共振特性，SERRS NP显示出高拉曼强度，但我们观察到非共振SERS NP浓度可以通过其独特发射的强度来定量，并且精度更高。五重混合物的光谱分解表明，当向混合物中引入更多组分时，与近红外共振SERRS NP口味相比，所研究的非共振SERS NP口味更能稳定地保持其检测限。
Multiplexed imaging, which allows for the interrogation of multiple molecular features simultaneously, is vital for addressing numerous challenges across biomedicine. Optically unique surface-enhanced Raman scattering (SERS) nanoparticles (NPs) have the potential to serve as a vehicle to achieve highly multiplexed imaging in a single acquisition, which is non-destructive, quantitative, and simple to execute. When using laser excitation at 785 nm, which allows for a lower background from biological tissues, near infrared (NIR) dyes can be used as Raman reporters to provide high Raman signal intensity due to the resonance effect. This class of imaging agents are known as surface-enhanced resonance Raman scattering (SERRS) NPs. Investigators have predominantly utilized two classes of Raman reporters in their nanoparticle constructs for use in biomedical applications: NIR-resonant and non-resonant Raman reporters. Herein, we investigate the multiplexing potential of five non-resonant SERS: BPE, 44DP, PTT, PODT, and BMMBP, and five NIR resonant SERRS NP flavors with heptamethine cyanine dyes: DTTC, IR-770, IR-780, IR-792, and IR-797, which have been extensively used for biomedical imaging applications. Although SERRS NPs display high Raman intensities, due to their resonance properties, we observed that non-resonant SERS NP concentrations can be quantitated by the intensity of their unique emissions with higher accuracy. Spectral unmixing of five-plex mixtures revealed that the studied non-resonant SERS NPs maintain their detection limits more robustly as compared to the NIR resonant SERRS NP flavors when introducing more components into a mixture.