Separation and size distribution of red blood cells of diverse size, shape, and origin by flow/hyperlayer field-flow fractionation.
Separation and size distribution of red blood cells of diverse size, shape, and origin by flow/hyperlayer field-flow fractionation.
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DOI：
10.1006/abio.1993.1287
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发表时间：
1993-07
影响因子：
2.9
通讯作者：
B. N. Barman;E. Ashwood;J. Giddings
中科院分区：
文献类型：
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作者：
B. N. Barman;E. Ashwood;J. Giddings
来自人、马、犬、猫和牛全血样品的红细胞（RBC）已经通过高速流/超层流场流分级分离（FI/HyFFF）分离和表征。通过该方法进行的基于洗脱的RBC分离主要基于细胞颗粒的大小和形状。红细胞的典型分离时间小于3分钟。使用基于均匀聚苯乙烯珠保留数据的校准图，可以从细胞样品的分形图中获得粒度分布。该方法被证明是有效的分离和表征细胞群，包括那些与细胞的异常形状和大小。为了研究细胞形态的差异，监测了500，000 Da葡聚糖诱导的形状和大小变化。F1/HyFFF洗脱曲线的变化表明，在葡聚糖的存在下，RBC的尺寸减小，但变得部分聚集。发现这些变化取决于聚合物浓度和特定的血液样品。将Fl/HyFFF的一些结果与Coulter计数器和显微镜的结果进行了比较。
Red blood cells (RBCs) from human, equine, canine, feline, and bovine whole blood samples have been separated and characterized by high-speed flow/hyperlayer field-flow fractionation (Fl/HyFFF). The elution-based separation of RBCs by this method is based mainly on the size and shape of the cell particles. The typical separation time for RBCs is less than 3 min. Size distributions can be derived from the fractograms of cell samples using a calibration plot based on retention data for uniform polystyrene beads. The method is shown to be effective both to separate and to characterize cell populations, including those with cells of abnormal shape and size. In order to investigate differences in cell morphology, shape and size changes induced by 500,000 Da Dextran were monitored. The changes in the Fl/HyFFF elution profiles indicate that the RBCs decrease in size but become partially aggregated in the presence of the dextran. These changes were found to depend on polymer concentration and specific blood samples. Some of the results from Fl/HyFFF were compared with those from the Coulter counter and from microscopy.