Genotype-phenotype linkage for directed evolution and screening of combinatorial protein libraries.
Genotype-phenotype linkage for directed evolution and screening of combinatorial protein libraries.
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用于组合蛋白质库的定向进化和筛选的基因型-表型联动。
DOI：
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发表时间：
2001
影响因子：
1.8
通讯作者：
H. Yanagawa
中科院分区：
文献类型：
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作者：
N. Doi;H. Yanagawa
用于定向蛋白质进化和功能基因组学领域研究的肽和蛋白质库筛选技术以惊人的速度发展。为了筛选功能性蛋白质，需要三种技术：（i）基因库（基因型）的构建，（ii）每个蛋白质（表型）与其编码基因（基因型）之间建立联系，以及（iii）从库中选择所需的蛋白质（表型）。本综述重点介绍了基因型-表型连锁技术，可分为三类：即细胞型联动、病毒型联动、阵列型联动方式。总结了这些方法，并讨论了它们的优缺点。
The technologies for screening peptide and protein libraries for studies in the fields of directed protein evolution and functional genomics have advanced with astonishing speed. For screening of functional proteins, three technologies are required: (i) the construction of a gene library (genotype), (ii) the establishment of a linkage between each protein (phenotype) and its encoding gene (genotype), and (iii) the selection of desired proteins (phenotype) from the library. This review highlights the genotype-phenotype linkage technologies, which can be classified into three types; that is, cell-type linkage, virus-type linkage, and array-type linkage methods. These methods are summarized, and their advantages and disadvantages are discussed.