PERITUBULAR CELL INFLUENCE ON THE EFFICIENCY OF ANDROGEN-BINDING PROTEIN SECRETION BY SERTOLI CELLS IN CULTURE
PERITUBULAR CELL INFLUENCE ON THE EFFICIENCY OF ANDROGEN-BINDING PROTEIN SECRETION BY SERTOLI CELLS IN CULTURE
复制标题
DOI：
10.1210/endo-108-4-1362
复制
发表时间：
1981-01-01
期刊：
影响因子：
4.8
通讯作者：
STOCCO, DM
中科院分区：
文献类型：
--
作者：
HUTSON, JC;STOCCO, DM
在体外培养的大鼠睾丸支持细胞中加入管周细胞，可提高其分泌雄激素结合蛋白（ABP）的效率。加入培养物中的管周细胞数量与ABP分泌量呈正相关。在用高度纯化的促卵泡激素处理的培养物或未处理的培养物中观察到这些反应。在暴露于支持细胞之前已经暴露于管周细胞的培养基没有模拟当管周细胞直接加入支持细胞时观察到的效果。支持细胞分泌ABP的效率不是平板接种密度的函数，因为每mg细胞蛋白的ABP量在支持细胞培养物中没有显著变化，而支持细胞培养物中存在的细胞数量变化很大。管周细胞在调节ABP分泌中发挥作用，因此支持细胞间通讯存在于生精小管的这两种细胞类型之间的假设。
The efficiency of androgen-binding protein (ABP) secretion into the medium of cultured rat Sertoli cells was increased by the addition of peritubular cells into the culture system. There was a positive correlation between the number of peritubular cells added to the cultures and the amount of ABP secreted. These responses were observed in cultures treated with highly purified follicle stimulating hormone or in untreated cultures. Media that had been exposed to peritubular cells before exposure to Sertoli cells did not mimic the effect observed when peritubular cells were added directly to Sertoli cells. The efficiency of ABP secretion by Sertoli cells was not a function of plating density, since the amount of ABP per mg cellular protein did not vary significantly among cultures of Sertoli cells which contained a wide variation in the numbers of cells present. Peritubular cells play a role in regulating ABP secretion and thus support the hypothesis that cell to cell communication exists between these 2 cell types of the seminiferous tubule.