Approaches to Characterize and Quantify Oligomerization of GPCRs.
Approaches to Characterize and Quantify Oligomerization of GPCRs.
复制标题
GPCR 寡聚化的表征和定量方法。
DOI：
10.1007/978-1-4939-2914-6_7
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Marsango S
中科院分区：
文献类型：
--
作者：
Marsango S
Fluorescence resonance energy transfer (FRET) is an approach widely used to detect protein–protein interactions in live cells. This approach is based on the sensitization of an “acceptor” molecule by the energy transfer from a “donor” when there is an overlap between the emission spectrum of the “donor” and the excitation spectrum of the “acceptor” and close proximity between the two fluorophore species (in the region of 8 nm). Various methods exist to quantify FRET signals: here, we describe the application of homogeneous time-resolved FRET (htrFRET) combined with Tag-lite™ technology and its application to determine not only protein–protein interactions but also the capability of GPCR mutant variants to form homomers compared to the wild type GPCR within the plasma membrane of transfected cells.
影响因子：
4.8
作者：
Kuszak, Adam J.;Pitchiaya, Sethuramasundaram;Sunahara, Roger K.
通讯作者：
Sunahara, Roger K.
影响因子：
4.8
作者：
Alvarez-Curto, Elisa;Ward, Richard J.;Milligan, Graeme
通讯作者：
Milligan, Graeme
影响因子：
4.8
作者：
Whorton, Matthew R.;Jastrzebska, Beata;Sunahara, Roger K.
通讯作者：
Sunahara, Roger K.