The in vivo and in vitro approaches for establishing a link between advanced glycation end products and lung cancer
The in vivo and in vitro approaches for establishing a link between advanced glycation end products and lung cancer
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DOI：
10.1002/jcb.27170
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发表时间：
2018-11-01
影响因子：
4
通讯作者：
Ahmad, Saheem
中科院分区：
文献类型：
--
作者：
Khan, Hamda;Khan, Mohd. Sajid;Ahmad, Saheem
晚期糖基化终产物（AGEs）与第三种衰老相关疾病直接相关，如心血管疾病、动脉硬化和神经变性。同样，这些不可逆和非酶促产物已被报道参与恶性癌症的进展。总的来说，与衰老相关的疾病和癌症的起源已经成为多年来人们感兴趣的主题。关于AGEs在癌症中的作用的研究很少在细胞系上进行。此外，过去在糖基化生物学领域的研究仍然缺乏对癌细胞体内和体外方法的了解。因此，我们的目标是关注并建立癌症和糖基化之间的联系，就所有可能的AGEs而言。在我们的研究中，糖基化动物模型中，羧甲基赖氨酸（CML）的含量增加了50.94%，而在癌症动物模型中，CML的含量比阴性对照组增加了45.94%。同样，我们也检测了荧光AGEs，发现糖基化动物模型和癌症动物模型中的AGEs分别比对照组增加了65.3%和58.63%。在糖基化和癌症动物模型中，蛋白质羰基含量也被发现增加。在我们的研究中，我们发现体外癌细胞模型中活性氧的水平也比对照组显著增加。这一初步突破表明AGEs存在于癌症和糖基化动物模型的血清中。
Advanced glycation end products (AGEs) are directly related to third aging-associated diseases, such as cardiovascular diseases, arteriosclerosis, and neurodegeneration. Likewise, these irreversible and nonenzymatic products have been reported to be involved in the progression of malignant cancers. In general, aging-associated diseases and the initiation of cancer have been subjects of interest for several years. Few studies on the role of AGEs in cancer have been performed on cell lines. Moreover, past investigations in the field of glycation biology still lack the knowledge of in vivo and in vitro approaches for cancer cells. Accordingly, we aimed to focus on and establish a link between cancer and glycation with respect to all the possible AGEs. In our study, the levels of carboxymethyllysine (CML) increased by 50.94% in an animal model of glycation, whereas in an animal model of cancer, the contents of CML increased by 45.94% compared with their negative controls. Similarly, fluorescent AGEs were also examined and were found to be increased by 65.3% and 58.63% in the animal models of glycation and cancer, respectively, compared with the control subjects. The protein carbonyl contents were also found to be enhanced in the animal models of glycation and cancer. In our study, the levels of reactive oxygen species were also found to be significantly increased in the in vitro model of cancer cells as compared with the controls. Such an initial breakthrough indicated that AGEs were present in the serum of the animal models of cancer and glycation.