The use of high-hydrostatic pressure treatment to decellularize blood vessels
The use of high-hydrostatic pressure treatment to decellularize blood vessels
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DOI：
10.1016/j.biomaterials.2010.01.073
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发表时间：
2010-05-01
期刊：
影响因子：
14
通讯作者：
Kishida, Akio
中科院分区：
文献类型：
--
作者：
Funamoto, Seiichi;Nam, Kwangwoo;Kishida, Akio
描述了使用高静水压（HHP）技术（>600 MPa）的脱细胞方法。HHP会破坏组织内的细胞。细胞碎片可以通过简单的洗涤过程消除，产生清洁的脱细胞组织。本研究采用HHP对猪主动脉进行脱细胞处理。评价了脱细胞组织的力学性能和体内性能。脱细胞组织的机械性能没有被HHP处理改变。异种移植实验证实了脱细胞组织的炎症减少。同种异体移植研究表明，脱细胞血管耐受动脉血压，管腔表面无凝块形成。此外，在植入后4周观察到细胞浸润到血管壁中，表明HHP处理可以作为高质量的脱细胞方法广泛应用。(C)2010爱思唯尔有限公司版权所有。
A decellularization method using high-hydrostatic pressure (HHP) technology (>600 MPa) is described. The HHP disrupts the cells inside the tissue. The cell debris can be eliminated with a simple washing process, producing clean, decellularized tissue. In this study, porcine aortic blood vessel was decellularized by HHP. The mechanical properties and in vivo performance of the decellularized tissue were evaluated. Mechanical properties of the decellularized tissue were not altered by the HHP treatment. Reduced inflammation of the decellularized tissue was confirmed by xenogenic transplant experimentation. An allogenic transplantation study showed that decellularized blood vessel endured the arterial blood pressure, and there was no clot formation on the luminal surface. In addition, cellular infiltration into the vessel wall was observed 4 weeks after implantation, suggesting that HHP treatments could be applied widely as a high-quality decellularization method. (C) 2010 Elsevier Ltd. All rights reserved.