Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase
Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase
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DOI：
10.1126/science.272.5259.279
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发表时间：
1996-04-12
期刊：
影响因子：
56.9
通讯作者：
Ohya, Y
中科院分区：
文献类型：
--
作者：
Qadota, H;Python, CP;Ohya, Y
1，3-β-D-葡聚糖合酶[也称为β（1->3）葡聚糖合酶]是一种多酶复合物，催化1，8-β-连接葡聚糖的合成，1，8-β-连接葡聚糖是酵母细胞壁的主要结构组分。温度敏感的突变体中的基本Rho型鸟苷三磷酸酶（GTAPK），Rho 1 p，显示热不稳定的葡聚糖合酶的活性，这是通过添加重组Rho 1 p恢复。表达组成型活性Rho 1 p的突变体的葡聚糖合酶不需要外源性三磷酸鸟苷的活性。Rho 1 p与β（1->3）葡聚糖合酶共纯化，并在体内与该复合物的Fks 1 p亚基结合。这两种蛋白质主要定位于细胞壁重塑的位点。因此，Rho 1 p似乎是β（1->3）葡聚糖合酶的调节亚基。
1,3-beta-D-Glucan synthase [also known as beta(1-->3)glucan synthase] is a multi-enzyme complex that catalyzes the synthesis of 1,8-beta-linked glucan, a major structural component of the yeast cell wall. Temperature-sensitive mutants in the essential Rho-type guanosine triphosphatase (GTPase), Rho1p, displayed thermolabile glucan synthase activity, which was restored by the addition of recombinant Rho1p. Glucan synthase from mutants expressing constitutively active Rho1p did not require exogenous guanosine triphosphate for activity. Rho1p copurified with beta(1-->3)glucan synthase and associated with the Fks1p subunit of this complex in vivo. Both proteins were localized predominantly at sites of cell wall remodeling. Therefore, it appears that Rho1p is a regulatory subunit of beta(1-->3)glucan synthase.