Feasibility of the assessment of cholesterol crystals in human macrophages using micro optical coherence tomography.
Feasibility of the assessment of cholesterol crystals in human macrophages using micro optical coherence tomography.
复制标题
DOI：
10.1371/journal.pone.0102669
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Tearney GJ
中科院分区：
文献类型：
--
作者：
Kashiwagi M;Liu L;Chu KK;Sun CH;Tanaka A;Gardecki JA;Tearney GJ
The presence of cholesterol crystals is a hallmark of atherosclerosis, but until recently, such crystals have been considered to be passive components of necrotic plaque cores. Recent studies have demonstrated that phagocytosis of cholesterol crystals by macrophages may actively precipitate plaque progression via an inflammatory pathway, emphasizing the need for methods to study the interaction between macrophages and crystalline cholesterol. In this study, we demonstrate the feasibility of detecting cholesterol in macrophages in situ using Micro-Optical Coherence Tomography (µOCT), an imaging modality we have recently developed with 1-µm resolution. Macrophages containing cholesterol crystals frequently demonstrated highly scattering constituents in their cytoplasm on µOCT imaging, and µOCT was able to evaluate cholesterol crystals in cultured macrophage cells. Our results suggest that µOCT may be useful for the detection and characterization of inflammatory activity associated with cholesterol crystals in the coronary artery.
登录
查看更多内容
影响因子：
39.3
作者：
Raffel, Owen Christopher;Merchant, Faisal M.;Jang, Ik-Kyung
通讯作者：
Jang, Ik-Kyung
影响因子：
39.3
作者：
Schaar, JA;Muller, JE;van der Steen, AFW
通讯作者：
van der Steen, AFW
影响因子：
3.7
作者：
Rajamäki K;Lappalainen J;Oörni K;Välimäki E;Matikainen S;Kovanen PT;Eklund KK
通讯作者：
Eklund KK
影响因子：
3.6
作者：
Liu, Linbo;Liu, Cheng;Chen, Nanguang
通讯作者：
Chen, Nanguang
影响因子：
24
作者：
Virmani, R;Burke, AP;Kolodgie, FD
通讯作者：
Kolodgie, FD