Identification of androgen-responsive genes in the rat ventral prostate by complementary deoxyribonucleic acid subtraction and microarray
Identification of androgen-responsive genes in the rat ventral prostate by complementary deoxyribonucleic acid subtraction and microarray
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DOI：
10.1210/en.2002-220718
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发表时间：
2003-04-01
期刊：
影响因子：
4.8
通讯作者：
Wang, Z
中科院分区：
文献类型：
--
作者：
Jiang, F;Wang, Z
我们已经报道了大约二十几个雄激素反应基因的鉴定，其诱导的基础上，雄激素在去势大鼠腹侧前列腺，使用基于PCR的消减杂交。相同的前列腺cDNA样品进行了改良的消减杂交，导致在21个新的雄激素反应基因，其中14个是已知的，7个是新的基因的鉴定。为了补充我们的减法研究，我们使用了Incyte大鼠cDNA微阵列，由8951个已知基因和表达的序列标签组成，发现162个基因被上调，143个基因被下调2.3倍或更多的雄激素。正如预期的那样，从我们的减法分离的基因与微阵列发现的基因重叠。对所有通过减法鉴定的基因和从微阵列中选择的少数基因进行北方印迹。所有检测的基因都受雄激素的调节，验证了我们的减法和微阵列研究。已鉴定的基因可分为几个功能组，包括代谢、蛋白质伴侣和运输、蛋白质合成、分泌、细胞周期和凋亡、结构和细胞外基质蛋白以及新蛋白。这些雄激素反应基因的鉴定将有助于了解雄激素在正常和癌性前列腺中的作用。
We have reported the identification of approximately two dozen androgen-responsive genes, on the basis of their induction by androgens in the castrated rat ventral prostate, using PCR-based subtractive hybridization. The same prostatic cDNA samples were subjected to a modified subtractive hybridization, resulting in the identification of 21 new androgen-responsive genes, of which 14 were known and 7 were novel genes. To complement our subtraction study, we have used an Incyte rat cDNA microarray, consisting of 8951 known genes and expressed sequence tags, and found that 162 genes were up-regulated and 143 genes were down-regulated 2.3-fold or more by androgens. As expected, the genes isolated from our subtraction overlap with genes found with microarray. Northern blot was carried out on all of the genes identified by subtraction and a few selected genes from microarray. All of the assayed genes are regulated by androgens, validating our subtraction and microarray studies. The identified genes can be classified into several functional groups, including metabolism, protein chaperoning and trafficking, protein synthesis, secretions, cell cycle and apoptosis, structural and extracellular matrix proteins, and novel proteins. Identification of these androgen-responsive genes will contribute to the understanding of androgen action in the normal and cancerous prostate.