RECONSTITUTION OF AN OPERATIONAL MHC CLASS-II COMPARTMENT IN NONANTIGEN-PRESENTING CELLS
RECONSTITUTION OF AN OPERATIONAL MHC CLASS-II COMPARTMENT IN NONANTIGEN-PRESENTING CELLS
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DOI：
10.1126/science.7985028
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发表时间：
1994-12-02
期刊：
影响因子：
56.9
通讯作者：
PETERSON, PA
中科院分区：
文献类型：
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作者：
KARLSSON, L;PELERAUX, A;PETERSON, PA
专业抗原呈递细胞（APCs）有一个独特的隔室，其中II类分子被提议获得抗原肽。遗传证据表明，人类白细胞抗原(HLA)-DM，一种罕见的II类分子，参与了这一过程。在非专业APCs中，通过转染II类、不变链（II）和H-2M（相当于小鼠DM）来重建肽获取。H-2M异二聚体出现在内体腔室中，而不是细胞表面，并且定位与II无关。所提供的数据表明，H-2M、II类和II类是有效形成稳定的II类肽复合物的最低要求成分，因此也是功能性II类隔室的最低要求成分。
Professional antigen-presenting cells (APCs) have a distinct compartment in which class II molecules are proposed to acquire antigenic peptides. Genetic evidence suggests that human leukocyte antigen (HLA)-DM, an unusual class II molecule, participates in this process. Peptide acquisition was reconstituted in nonprofessional APCs by transfection of class II, invariant chain (Ii), and H-2M, the murine equivalent of DM. The H-2M heterodimer appeared in an endosomal compartment, not at the cell surface, and the localization was independent of Ii. The data presented show that H-2M, class II, and Ii are the minimally required components for efficient formation of stable class II-peptide complexes, and thus for a functional class II compartment.