Breath isoprene excretion during rest and low-intensity cycling exercise is associated with skeletal muscle mass in healthy human subjects
Breath isoprene excretion during rest and low-intensity cycling exercise is associated with skeletal muscle mass in healthy human subjects
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DOI：
10.1088/1752-7163/abbf39
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发表时间：
2021-01-01
影响因子：
3.8
通讯作者：
Hotta, Norio
中科院分区：
文献类型：
--
作者：
Hori, Amane;Suijo, Kenichi;Hotta, Norio
异戊二烯是呼出气中所含的许多内源性挥发性有机化合物之一，其生理作用尚不清楚。近年来，呼出的异戊二烯与骨骼肌有关。一些研究表明，骨骼肌产生和/或储存一些异戊二烯。然而，支持这种关联的证据仍然稀少且不确定。此外，由于骨骼肌数量和质量的变化，衰老可能会影响呼吸异戊二烯反应。因此，我们研究了年轻（n = 7）和老年（n = 7）成人呼吸异戊二烯排泄（（V）超过点（异戊二烯））和骨骼肌质量之间的关联。参与者在休息3分钟后进行18分钟的自行车运动。根据Karvonen公式计算，工作负荷相当于心率储备的30%。在运动测试期间收集每个参与者的呼出气。我们从每分钟通气量和异戊二烯浓度的乘积计算出（V）over dot（异戊二烯），然后研究（V）over dot（异戊二烯）和肌肉质量之间的关系，这是通过多频生物电阻抗分析测量的。重要的是，肌肉质量仍然是一个重要的决定因素，即使在调整年龄后，也可以解释休息时（V）对点（异戊二烯）的变化。此外，无论年龄大小，肌肉质量是运动期间（V）过点（异戊二烯）反应的重要决定因素。这些数据表明，骨骼肌质量可能是在休息和运动反应期间（V）超过点（异戊二烯）的决定因素之一。因此，我们认为骨骼肌可能在产生和/或储存一些内源性异戊二烯中发挥重要作用。这些新知识将有助于更好地了解异戊二烯在人体中的生理功能（批准号20190079）。
The physiological roles of isoprene, which is one of the many endogenous volatile organic compounds contained in exhaled breath, are not well understood. In recent years, exhaled isoprene has been associated with the skeletal muscle. Some studies have suggested that the skeletal muscle produces and/or stores some of the isoprene. However, the evidence supporting this association remains sparse and inconclusive. Furthermore, aging may affect breath isoprene response because of changes in the skeletal muscle quantity and quality. Therefore, we investigated the association between the breath isoprene excretion ((V) over dot(isoprene)) and skeletal muscle mass in young (n = 7) and old (n = 7) adults. The participants performed an 18 min cycling exercise after a 3 min rest. The workload corresponded to an intensity of 30% of the heart rate reserve, as calculated by the Karvonen formula. The exhaled breath of each participant was collected during the exercise test. We calculated (V) over dot(isoprene) from the product minute ventilation and isoprene concentration and, then, investigated the relationships between (V) over dot(isoprene) and muscle mass, which was measured by multi-frequency bioelectrical impedance analysis. Importantly, muscle mass persisted as a significant determinant that explained the variance in (V) over dot(isoprene) at rest even after adjusting for age. Furthermore, the muscle mass was a significant determinative factor for (V) over dot(isoprene) response during exercise, regardless of age. These data indicated that skeletal muscle mass could be one of the determinative factors for (V) over dot(isoprene) during rest and response to exercise. Thus, we suggest that the skeletal muscle may play an important role in generating and/or storing some of the endogenous isoprene. This new knowledge will help to better understand the physiological functions of isoprene in humans (Approval No. 20190079).