HEPATIC GLYCOGEN PATTERNS IN FASTED AND FED RATS
HEPATIC GLYCOGEN PATTERNS IN FASTED AND FED RATS
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DOI：
10.1002/aja.1001400302
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发表时间：
1974-01-01
影响因子：
--
通讯作者：
CARDELL, RR
中科院分区：
文献类型：
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作者：
BABCOCK, MB;CARDELL, RR
描述了适应精确控制的喂养时间表的大鼠的肝糖原模式和随意喂养的大鼠。在禁食22小时和进餐2小时后，以精确的间隔对肝脏样本进行生化和组织化学糖原分析。冷冻替代后糖原组织化学检测显示肝糖原呈小叶状，与化学测定的糖原水平和大鼠的营养状态有关。禁食22小时后，低糖原水平(0.09%)的大鼠肝细胞未见明显染色。在对照饲养的大鼠中，摄食导致整个小叶的糖原沉积，但在整个糖原积累的早期阶段，糖原的中心浓度最高。随着糖原沉积的继续，在高糖原水平(>；5%)的大鼠中观察到门静脉周围小叶图案。糖原耗竭减少了整个小叶细胞中的糖原染色，但小叶中心模式占主导地位，直到肝门周围模式出现的耗竭后期。自由采食大鼠的肝糖原形态相似，但糖原最大沉积以小叶中心甚至小叶分布为特征，肝门周围糖原形态仅在极端禁食的大鼠中罕见。随意喂养的大鼠和对照喂养的大鼠之间小叶模式的差异显然与随意喂养的动物达到的最大肝糖原水平较低有关。
Hepatic glycogen patterns are described for rats adapted to a precisely controlled feeding schedule and ad libitum fed rats. Liver samples were processed for biochemical and histochemical glycogen analysis at precise intervals following a 22 hour fast and a 2 hour meal. Histochemical determination of glycogen (PAS) after freeze substitution showed lobular patterns of hepatic glycogen which correlate with chemically determined glycogen levels and nutritional states of the rats. After 22 hour fasting, hepatocytes from rats with low glycogen levels (< 0.09%) exhibited no significant staining. In control fed rats, feeding caused glycogen deposition throughout the lobule but in greatest concentration centrilobularly throughout the early phases of glycogen accumulation. As glycogen deposition continued, periportal lobular patterns were observed in rats with high glycogen levels (> 5%). Glycogen depletion reduced glycogen staining in cells throughout the lobule, but centrilobular patterns prevailed until late in depletion when periportal patterns appeared. Ad libitum‐fed rats showed similar glycogen patterns except maximum deposition was characterized by centrilobular or even lobular distribution of glycogen, and periportal patterns of glycogen were seen only rarely in extreme fasted rats. Differences in lobular patterns between ad libitum and control fed rats is apparently related to lower maximum hepatic glycogen levels reached by ad libitum‐fed animals.