CO2 Uptake and Fixation by a Thermoacidophilic Microbial Community Attached to Precipitated Sulfur in a Geothermal Spring
CO2 Uptake and Fixation by a Thermoacidophilic Microbial Community Attached to Precipitated Sulfur in a Geothermal Spring
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DOI：
10.1128/aem.02751-08
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发表时间：
2009-07-01
影响因子：
4.4
通讯作者：
Geesey, Gill G.
中科院分区：
文献类型：
--
作者：
Boyd, Eric S.;Leavitt, William D.;Geesey, Gill G.
在73摄氏度以上的温度下，光合作用的上限，碳固定是由化学合成嗜热菌进行的。怀俄明州的黄石国家公园（YNP）具有许多热特征，虽然太热而不利于光合作用，但可能支持基于化学合成的碳固定。据我们所知，在YNP或其他高温陆地地热泉的高温下，化学合成反应的原位速率尚未报道。在YNP诺里斯间歇泉盆地的龙泉（73 ℃，pH 3.1）源头，附着在沉淀元素硫（S-o絮凝物）上的微生物群落表现出最大的CO2吸收速率，为21.3 +/- 11.9 μ g C 10（7）细胞（-1）h（-1）。当外推超过估计的总数量的S-O絮体在春天的来源，S-O絮体相关的微生物群落占121毫克的C h（-1）在这个网站的吸收。在每个细胞的基础上，速率高于计算的光合垫微生物群落占主导地位的聚球藻属。在碱性温泉中的温度相当。在细胞核酸池中回收了由S-o絮凝物相关生物质以CO2形式吸收的一部分碳，表明吸收与固定偶联。最丰富的序列在16 S rRNA克隆库的S-O絮凝体相关的社会有关chemolithoautotrophic Hydrogenobaculum菌株以前分离的诺里斯间歇泉盆地。这些微生物可能有助于吸收和固定二氧化碳在这个地热栖息地。
Carbon fixation at temperatures above 73 degrees C, the upper limit for photosynthesis, is carried out by chemosynthetic thermophiles. Yellowstone National Park (YNP), Wyoming possesses many thermal features that, while too hot for photosynthesis, presumably support chemosynthetic-based carbon fixation. To our knowledge, in situ rates of chemosynthetic reactions at these high temperatures in YNP or other high-temperature terrestrial geothermal springs have not yet been reported. A microbial community attached to precipitated elemental sulfur (S-o floc) at the source of Dragon Spring (73 degrees C, pH 3.1) in Norris Geyser Basin, YNP, exhibited a maximum rate of CO2 uptake of 21.3 +/- 11.9 mu g of C 10(7) cells(-1) h(-1). When extrapolated over the estimated total quantity of S-o floc at the spring's source, the S-o floc-associated microbial community accounted for the uptake of 121 mg of C h(-1) at this site. On a per-cell basis, the rate was higher than that calculated for a photosynthetic mat microbial community dominated by Synechococcus spp. in alkaline springs at comparable temperatures. A portion of the carbon taken up as CO2 by the S-o floc-associated biomass was recovered in the cellular nucleic acid pool, demonstrating that uptake was coupled to fixation. The most abundant sequences in a 16S rRNA clone library of the S-o floc-associated community were related to chemolithoautotrophic Hydrogenobaculum strains previously isolated from springs in the Norris Geyser Basin. These microorganisms likely contributed to the uptake and fixation of CO2 in this geothermal habitat.