Roles of Planar Cell Polarity Signaling in Maturation of Neuronal Precursor Cells in the Postnatal Mouse Olfactory Bulb
Roles of Planar Cell Polarity Signaling in Maturation of Neuronal Precursor Cells in the Postnatal Mouse Olfactory Bulb
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DOI：
10.1002/stem.1137
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发表时间：
2012-08-01
期刊：
影响因子：
5.2
通讯作者：
Sawamoto, Kazunobu
中科院分区：
文献类型：
--
作者：
Hirota, Yuki;Sawada, Masato;Sawamoto, Kazunobu
室下区 (SVZ) 干细胞产生的神经元前体细胞迁移并分化为嗅球 (OB) 中的成熟中间神经元。在此过程中细胞动态形态变化的机制很大程度上是未知的。 Wnt/平面细胞极性 (PCP) 信号传导调节各种发育事件，包括神经元迁移和神经突形成。在这里，我们研究了产后小鼠 OB 新生神经元中 PCP 通路的两个组成部分 Dishevelled2 和 Van Gogh like-2 的功能。通过电穿孔或慢病毒介导将携带这些基因的敲低或显性失活构建体的载体引入SVZ，改变了OB中新生神经元的分布和树突形成，表明PCP信号传导参与调节OB中新神经元的成熟。干细胞 2012；30:1726-1733
Neuronal precursor cells generated by stem cells in the subventricular zone (SVZ) migrate and differentiate into mature interneurons in the olfactory bulb (OB). The mechanisms responsible for the dynamic morphological changes in cells during this process are largely unknown. Wnt/planar cell polarity (PCP) signaling regulates various developmental events, including neuronal migration and neurite formation. Here, we studied the function of two components of the PCP pathway, Dishevelled2 and Van Gogh like-2, in the newborn neurons in the postnatal mouse OB. Electroporation- or lentivirus-mediated introduction of vectors carrying a knockdown or dominant-negative construct of these genes into the SVZ altered the distribution and dendrite formation of newborn neurons in the OB, suggesting that PCP signaling is involved in regulating the maturation of new neurons in the OB. STEM CELLS 2012;30:1726-1733