Elucidation of structure-inhibition relationship of monosaccharides derived pseudo-lignin in enzymatic hydrolysis
Elucidation of structure-inhibition relationship of monosaccharides derived pseudo-lignin in enzymatic hydrolysis
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酶水解中单糖衍生假木质素的结构抑制关系的阐明
DOI：
10.1016/j.indcrop.2018.01.046
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发表时间：
2018-03-01
影响因子：
5.9
通讯作者：
Yong, Qiang
中科院分区：
文献类型：
--
作者：
He, Juan;Huang, Caoxing;Yong, Qiang
假木质素是碳水化合物脱水合成、芳香化而形成的芳香物质。本文采用稀硫酸处理竹材中的单糖和综纤维素，制备假木质素，并分离出酸处理后的残余木质素。利用扫描电镜、红外光谱和核磁共振谱等无损技术分析了假木质素的结构信息。同时，以RL为对照，评价了假木质素和纤维素酶的非生产性吸附性能。结果表明，假木质素为可感液滴，由芳香族、羰基和芳香族结构组成。假木质素中脂肪族羟基和羧酸的含量分别为0.61-0.79 mmol/g和0.64-0.68 mmol/g。RL的总OH含量（3.51 mmol/g）高于假木质素（2.52-3.05 mmol/g）。由于假木质素具有较低的表面负电荷、疏水性、朗缪尔常数（K）和结合强度（R），其对酶的负面影响比RL弱。
Pseudo-lignin is defined as aromatic material, which could be formed through dehydration synthesis and aromatisation of carbohydrate. In this work, dilute sulfuric acid was carried out to treat monosaccharides and holocellulose to generate pseudo-lignin and the residual lignin (RL) in acid-pretreated bamboo was isolated. The structural informations of pseudo-lignin were assayed by nondestructive techniques (SEM, FTIR, and P-31 NMR). Meanwhile, the nonproductive adsorption performance of pseudo-lignin and cellulase was evaluated, taking RL as the comparison. Results showed that pseudo-lignin represented sensible droplets and was consist of aliphatics, carbonyl and aromatic structures. The amounts of aliphatic hydroxyl groups and carboxylic acid in pseudo-lignin were 0.61-0.79 mmol/g and 0.64-0.68 mmol/g, respectively. Total OH content of RL (3.51 mmol/g) was higher than pseudo-lignins (2.52-3.05 mmol/g). Due to the lower negative surface charge, hydrophobic nature, Langmuir constant (K) and binding strength (R) of pseudo-lignin, it represented weaker negative impact on enzymes than RL.