Comparison of replicase localization in different types of mouse hepatitis virus (MHV)-infected cells.
Comparison of replicase localization in different types of mouse hepatitis virus (MHV)-infected cells.
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DOI：
10.1007/978-1-4615-1325-4_43
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发表时间：
2001
影响因子：
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通讯作者：
R. Gosert;A. Kanjanahaluethai;D. Egger;K. Bienz;S. Baker
中科院分区：
文献类型：
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作者：
R. Gosert;A. Kanjanahaluethai;D. Egger;K. Bienz;S. Baker
The replication complex (RC) of virtually all positive strand RNA viruses has been shown to be intimately associated with cellular membranes. However, different RNA viruses seem to target or recruit distinct membranes for the assembly of their RCs. For example, poliovirus replicates its RNA on the surface of membranous vesicles which seem to evolve from the endo-plasmic reticulum (ER). The vesicles form a rosette-like structure (Bienzet al1992; Eggeret al1996). For tobacco etch potyvirus viral replication takes place at ER-derived vesicles and results in a collapse of the ER (Schaadet al1997). In contrast, alphaviruses appear to use the cytosolic surface of endocytic organelles for the formation of their RCs (Froshaueret al1988). For Nidoviruses such as arteriviruses and coronaviruses, the story is even more complex. Recently, it was shown that equine arteritis virus (EAV) generates ER-derived double-membrane vesicles (DMVs) (Pedersenet al1999). In contrast, studies of the Coronavirus mouse hepatitis virus (MHV) implicated a role for late endosomes in the formation of the RC (van der Meeret al1999). Recently, we have shown that translation products of the MHV replicase gene localized to different membrane structures in different cell lines. For a human cell line, viral replicase products colocalized with golgi markers, but in a murine cell line, the viral products and ER-derived membranes colocalized (Shiet al1999). To extend our studies, we wanted to determine the viral and cellular factor(s) that drive the generation of the MHV RC at distinct membranes in the different cell lines.
