Overexpression of the anaphylatoxin receptors, complement anaphylatoxin 3a receptor and complement anaphylatoxin 5a receptor, in the nasal mucosa of patients with mild and severe persistent allergic rhinitis
Overexpression of the anaphylatoxin receptors, complement anaphylatoxin 3a receptor and complement anaphylatoxin 5a receptor, in the nasal mucosa of patients with mild and severe persistent allergic rhinitis
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DOI：
10.1016/j.jaci.2008.04.028
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发表时间：
2008-07-01
影响因子：
14.2
通讯作者：
Lee, Sang Hag
中科院分区：
文献类型：
--
作者：
Jun, Sung Whan;Kim, Tae Hoon;Lee, Sang Hag
背景：虽然补体过敏毒素肽，补体过敏毒素3a （C3a）和补体过敏毒素5a （C5a）参与变应性鼻炎的炎症过程，但过敏粘膜和补体受体之间的直接相互作用尚未得到证实。目的：探讨C3a受体（C3aR）和C5a受体（C5aR）在正常、轻度和重度持续性变应性鼻黏膜中的表达水平和分布规律。方法：应用C3aR和C5aR抗体对正常鼻黏膜、轻度和重度持续性过敏鼻黏膜进行免疫组化和免疫印迹检测。结果：在正常和变应性鼻黏膜炎症细胞中均检测到补体过敏毒素5a受体，其在变应性鼻黏膜中的表达水平明显高于正常鼻黏膜。正常和过敏性鼻黏膜中的C3aR通常在非髓系细胞如上皮细胞、粘膜下腺和神经纤维中表达。C3aR在重度持续性变应性鼻黏膜海绵窦内皮及周围血管周围肌层中表达，而在正常和轻度变应性鼻黏膜中不表达。Western blot结果显示，C3aR在重度持续性过敏鼻黏膜中的表达水平较正常和轻度鼻黏膜明显升高。结论：根据C3aR和C5aR的位置，C5aR可能在变应性鼻黏膜，尤其是重度持续性变应性鼻黏膜中发挥炎症细胞失活作用，而C3aR可能介导黏液分泌和粘膜肿胀。
Background: Although the complement anaphylatoxin peptides, complement anaphylatoxin 3a (C3a) and complement anaphylatoxin 5a (C5a), are implicated in the inflammatory process in allergic rhinitis, a direct interaction between allergic mucosa and complement receptors has not been demonstrated.Objective: We investigated the expressional levels and distributional patterns of the C3a receptor (C3aR) and C5a receptor (C5aR) in normal, mild, and severe persistent allergic nasal mucosa.Methods: Immunohistochemistry and Western blotting using C3aR and C5aR antibodies were applied to normal nasal, mild, and severe persistent allergic nasal mucosa.Results: Complement anaphylatoxin 5a receptor was detected in the inflammatory cells of normal and allergic nasal mucosa, and its expression level was significantly higher in allergic nasal mucosa than normal nasal mucosa. C3aR in normal and allergic nasal mucosa was commonly expressed in nonmyeloid cells such as epithelial cells, submucosal glands, and nerve fibers. In addition, C3aR was expressed in the endothelium of cavernous sinuses and the surrounding perivascular muscle layer in severe persistent allergic nasal mucosa, but not in normal and mild allergic nasal mucosa. Western blotting demonstrated that the expression level of C3aR was significantly increased in severe persistent allergic nasal mucosa compared with normal and mild nasal mucosa.Conclusion: On the basis of the location of C3aR and C5aR, C5aR may play a role inactivation of inflammatory cells, whereas C3aR may mediate mucus secretion and mucosal swelling in allergic nasal mucosa, especially severe persistent allergic nasal mucosa.