SARS-CoV-2 RNA detection of hospital isolation wards hygiene monitoring during the Coronavirus Disease 2019 outbreak in a Chinese hospital
SARS-CoV-2 RNA detection of hospital isolation wards hygiene monitoring during the Coronavirus Disease 2019 outbreak in a Chinese hospital
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DOI：
10.1016/j.ijid.2020.04.024
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发表时间：
2020-05-01
影响因子：
8.4
通讯作者：
Qu, Tingting
中科院分区：
文献类型：
--
作者：
Wang, Jie;Feng, Haiting;Qu, Tingting
目的：监测浙江大学第一附属医院医院环境表面、污水及隔离病房医护人员个人防护用品中SARS-Cov-2的存在情况。方法：用1000mg /L含氯消毒剂常规擦拭物品表面。空气和污水按常规严格消毒。使用拭子对隔离病房工作人员的医院环境表面和个人防护装备进行取样。从不同的进水口和出水口抽取污水。采集患者呼吸道和粪便标本。隔离病房工作人员呼吸标本每周采集1次。采用实时定量反转录PCR （qRT-PCR）方法证实了SARS-Cov-2 RNA的存在。对SARS-Cov-2 RNA阳性样本进行病毒培养。结果：研究期间，有33例实验室确诊患者在医院隔离病房住院。隔离病房36份物品表面标本和9份工作人员个人防护用品标本均未检出SARS-Cov-2 RNA。前处理消毒池入口3份污水样本呈SARS-CoV-2 RNA阳性，前处理消毒池出口3份样本呈弱阳性，最后消毒池出口3份样本呈阴性。5份不同地点的污水样本经SARS-Cov-2病毒培养均为阴性。隔离病房工作人员呼吸道标本均无阳性。结论：该污水消毒池进水口SARS-Cov-2 RNA呈阳性，末次污水消毒池出水口SARS-Cov-2 RNA呈阴性，但培养未检出活病毒。本研究监测数据表明，严格的消毒和手部卫生可降低隔离病房工作人员的医院相关性COVID-19感染风险。(C) 2020作者。由爱思唯尔有限公司代表国际传染病学会出版。
Objectives: The aim of this paper was to monitor the presence of SARS-Cov-2 among hospital environment surfaces, sewage, and personal protective equipment (PPE) of staffs in isolation wards in the First Affiliated Hospital of Zhejiang University, China.Methods: Surfaces of objects were routinely wiped with 1000 mg/L chlorine containing disinfectant. Air and sewage disinfection was proceeded routinely and strictly. Hospital environmental surfaces and PPE of staffs in isolation wards were sampled using swabs. The sewage from various inlet and outlets were sampled. The respiratory and stool specimens of patients were collected. The respiratory specimens of staffs in the isolation wards were also sampled once a week. Quantitative real-time reverse transcription PCR (qRT-PCR) methods were used to confirm the existence of SARS-Cov-2 RNA. Viral culture was done for the samples positive for SARS-Cov-2 RNA.Results: During the study period, 33 laboratory-confirmed patients were hospitalized in isolation wards in the hospital. None of SARS-Cov-2 RNA was detected among the 36 objects surface samples and 9 staffs PPE samples in isolation wards. Though the 3 sewage samples from the inlet of preprocessing disinfection pool were positive for SARS-CoV-2 RNA and the sample from the outlet of preprocessing disinfection pool was weakly positive, the sewage sample from the outlet of the last disinfection pool was negative. All of the 5 sewage samples from various points were negative by viral culture of SARS-Cov-2. None of the respiratory specimens of staffs in the isolation wards were positive.Conclusions: Though SARS-Cov-2 RNA of the sewage samples were positive from inlets of the sewage disinfection pool and negative from the outlet of the last sewage disinfection pool, no viable virus was detected by culture. The monitoring data in this study suggested that the strict disinfection and hand hygiene could decrease the hospital-associated COVID-19 infection risk of the staffs in isolation wards. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.