Presumption of toxic mechanism of tributyltin on photosystem in marine diatoms by comparison to diuron as a reference agent through chlorophyll a fluorescence transient analysis
Presumption of toxic mechanism of tributyltin on photosystem in marine diatoms by comparison to diuron as a reference agent through chlorophyll a fluorescence transient analysis
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通过叶绿素a荧光瞬态分析与敌草隆对照推测三丁基锡对海洋硅藻光系统的毒性机制
DOI：
10.11403/jset.22.13
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Y Oshima
中科院分区：
文献类型：
--
作者：
MRM Khanam;Y Shimasaki;M Tsuyama;H Goto;X Qiu;K Mukai;Y Oshima
在这项研究中，OJIP测试的两种海洋硅藻物种，海链藻（单细胞物种）和海链藻复合体marinoi-dohrnii（链形成物种），暴露于敌草隆和三丁基锡（TBT）的EC 10和EC 50水平72小时。敌草隆暴露72 h后，两种植物在300 μs和J阶跃（2 ms）处的相对荧光强度（Ft/Fo值）均呈上升趋势，荧光曲线的初始斜率Mo值也显著增加（P< 0.05），而TBT对上述指标无明显影响。这些结果表明敌草隆有效地阻断了光系统II在次级电子受体QB上催化的光合电子传递，这是敌草隆在硅藻中的原始生物活性。TBT处理后，某些指标（如PGE_0、PGE_0、Fv/Fo、PIABS）显著降低（P< 0.05）。虽然造成这种降低的机制尚不清楚，但与敌草隆处理相比，三丁基锡化合物处理使Fv/Fo（水分解活性的一个指标）水平相对严重地降低，这表明三丁基锡化合物通过抑制光合放氧系统来抑制光合功能，这是一种与敌草隆不同的机制。此外，本研究表明，PIABS来源于OJIP测试是一个高灵敏度的生物标记检测污染物的毒性效应，抑制光合功能。
In this study, an OJIP-test of two marine diatom species, Thalassiosira pseudonana (single-celled species) and Skeletonema marinoi-dohrnii complex (chain-forming species), were exposed to EC10 and EC50 levels of diuron and tributyltin (TBT) for 72 h. Increased tendency of relative fluorescence intensity (Ft/Fo value) at 300 μs and J step (2 ms) and a significantly (P< 0.05) increased Mo value (initial slope of fluorescence curve) were observed during 72 of diuron exposure in both species, whereas TBT did not increase those. These results strongly suggest that diuron efficiently blocked photosystem II-catalyzed photosynthetic electron transport at the secondary electron acceptor QB, which is original biological activity of diuron in diatoms. However, some parameters (eg, ϕPo and ϕEo, Fv/Fo and PIABS,) were significantly (P< 0.05) decreased by TBT treatment. Although the mechanism responsible for the decrease is not clear, relatively severe reductions in levels of Fv/Fo (an indicator of water-splitting activity) by TBT treatment than diuron treatment suggest that TBT inhibits photosynthetic function via inhibition of photosynthetic oxygen evolving systems, a different mechanism than that of diuron. Moreover, present study suggested that PIABS derived from OJIP-test is a high sensitive biological marker for detecting the toxic effect of pollutants which inhibit photosynthetic function.
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影响因子：
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影响因子：
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