Genetic analysis of a morphological shape difference in the male genitalia of Drosophila simulans and D. mauritiana.
Genetic analysis of a morphological shape difference in the male genitalia of Drosophila simulans and D. mauritiana.
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DOI：
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发表时间：
1996-04
期刊：
影响因子：
3.3
通讯作者：
Jianjun Liu;John M. Mercer;L. F. Stam;Gregory C. Gibson;Zhao-Bang Zeng;Cathy C. Laurie
中科院分区：
文献类型：
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作者：
Jianjun Liu;John M. Mercer;L. F. Stam;Gregory C. Gibson;Zhao-Bang Zeng;Cathy C. Laurie
果蝇的两个密切相关的物种，D. simulans 和 D. mauritiana，在雄性生殖弓后叶的形态上存在显着差异。波瓣变化的大小和形状方面都可以通过基于椭圆傅里叶和主成分分析的形态测量描述符来量化。通过杂交自交系来研究该数量性状 (PC1) 的遗传结构，产生两个回交群体，每个回交群体大约有 200 个个体，并在 18 个标记位点的帮助下通过复合区间作图程序对其进行联合分析。亲本系在 PC1 上显示出很大的差异（30.4 环境标准偏差），并且标记占回交群体中 > 80% 的表型变异。分析的 15 个区间中的 8 个显示了数量性状位点 (QTL) 的令人信服的证据，估计 QTL 效应的范围是亲本差异的 5.7-15.9%（1.7-4.8 环境标准差）。这些估计值可能代表单个区间（平均长度为 23 cM）内多个 QTL 的联合效应。尽管有一些证据表明毛里求斯等位基因具有部分优势并且具有上位性，但遗传模式很大程度上是累加性的。
Two closely related species of Drosophila, D. simulans and D. mauritiana, differ markedly in morphology of the posterior lobe of the male genital arch. Both size and shape aspects of lobe variation can be quantified by a morphometric descriptor based on elliptical Fourier and principal components analyses. The genetic architecture of this quantitative trait (PC1) was investigated by hybridizing inbred lines to produce two backcross populations approximately 200 individuals each, which were analyzed jointly by a composite interval mapping procedure with the aid of 18 marker loci. The parental lines show a large difference in PC1 (30.4 environmental standard deviations), and the markers account for > 80% of the phenotypic variation in backcross populations. Eight of 15 intervals analyzed show convincing evidence of quantitative trait loci (QTL), and the range of estimated QTL effects is 5.7-15.9% of the parental difference (1.7-4.8 environmental standard deviations). These estimates may represent the joint effects of multiple QTL within a single interval (which averaged 23 cM in length). Although there is some evidence of partial dominance of mauritiana alleles and for epistasis, the pattern of inheritance is largely additive.