The rab16B promoter of rice contains two distinct abscisic acid-responsive elements
The rab16B promoter of rice contains two distinct abscisic acid-responsive elements
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DOI：
10.1104/pp.112.2.483
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发表时间：
1996-10-01
期刊：
影响因子：
7.4
通讯作者：
Shimamoto, K
中科院分区：
文献类型：
--
作者：
Ono, A;Izawa, T;Shimamoto, K
为了定位脱落酸（阿坝）诱导的rab 16基因的表达，将rab 16 A启动子与编码β-葡萄糖醛酸酶的gusA报告基因连接，并导入水稻（Oryza sativa L.）植物rab 16 A启动子的活性在营养器官和花器官的各种组织中受到阿坝和渗透胁迫的诱导。在花药和胚中，rab 16 A启动子在阿坝缺乏的情况下是活跃的。为了阐明rab 16启动子的顺式元件赋予ABA诱导表达，将rab 16 B启动子的各种修饰的40-bp片段（-264至-225）融合到截短的（-46 bp）花椰菜花叶病毒35 S最小启动子，并分析它们在原生质体中的活性。瞬时分析表明，40 bp的片段由两个独立的ABA反应元件，基序I（AGTACGTGGC）和基序III（GCCGCGTGGC）。基序I和基序III都是阿坝诱导所必需的;然而，每一个都可以取代另一个。进一步的分析表明，这些基序III有一个独特的DNA序列特异性从基序I的ABA响应元件，这表明这两个基序在体内与不同的转录因子相互作用。
To localize abscisic acid (ABA)-inducible gene expression of rab16 genes, rab16A promoter was linked to the gusA reporter gene encoding p-glucuronidase and introduced into rice (Oryza sativa L.) plants. The activity of rab16A promoter was induced by ABA and osmotic stresses in various tissues of vegetative and floral organs. In anthers and embryos, rab16A promoter was active in the absence of ABA. To elucidate cis-elements of the rab16 promoter that confer ABA-inducible expression, variously modified 40-bp fragments (-264 to -225) of the rab16B promoter were fused to a truncated (-46 bp) cauliflower mosaic virus 35S minimal promoter, and their activities in protoplasts were analyzed. The transient assays revealed that the 40-bp fragment consists of two separate ABA-responsive elements, motif I (AGTACGTGGC) and motif III (GCCGCGTGGC). Motif I and motif III are both required for ABA induction; however, each can substitute for the other. Further analyses of these motifs indicated that motif III has a distinct DNA sequence specificity as an ABA-responsive element from motif I, suggesting that the two motifs interact with different transcription factors in vivo.