Biomarkers of Gut Microbiota in Chronic Spontaneous Urticaria and Symptomatic Dermographism.
Biomarkers of Gut Microbiota in Chronic Spontaneous Urticaria and Symptomatic Dermographism.
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慢性自发性荨麻疹和症状性皮肤病中肠道微生物群的生物标志物
DOI：
10.3389/fcimb.2021.703126
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发表时间：
2021
影响因子：
5.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Liu R;Peng C;Jing D;Xiao Y;Zhu W;Zhao S;Zhang J;Chen X;Li J
研究背景慢性荨麻疹（chronic urticaria，CU）是一种与Th2免疫应答相关的慢性炎症性皮肤病。CU的两种最常见的亚型，即，慢性自发性荨麻疹和症状性皮肤划痕症（CSD），往往并存。然而，CSD的发病机制尚不清楚。肠道微生物群在免疫相关的炎症性疾病中起着重要作用。本研究旨在探讨肠道菌群与CSD的相关性。方法采用病例对照研究方法，对CSD患者及性别、年龄匹配的正常对照进行研究。粪便样本的16S核糖体DNA测序用于检测所有受试者的肠道菌群。采用QPCR进一步验证两组间存在差异的种属。结果CSD患者肠道菌群α多样性降低，同时肠道菌群结构发生明显变化。受试者工作特征曲线（ROC）分析表明，CSD患者亚长粒菌和溴化瘤胃球菌明显减少，对CSD有潜在的诊断价值。肠杆菌科和克雷伯菌属与CSD的持续时间呈正相关，而梭菌disporicum与皮肤病生活质量指数（DLQI）呈正相关。结论CSD患者肠道菌群失衡。Subdoligranulum和Ruminococcus bromii是CSD的肠道微生物生物标志物。
Background Chronic urticaria (CU) is a chronic inflammatory skin disease associated with Th2 immune response. The two most common subtypes of CU, i.e., chronic spontaneous urticaria and symptomatic dermographism (CSD), often coexist. However, the pathogenesis of CSD is still unclear. Gut microbiota plays an important role in immune-related inflammatory diseases. The purpose of this study was to explore the correlation between gut microbiota and CSD. Methods A case-control study was conducted on CSD patients as well as gender- and age-matched normal controls (NCs). The 16S ribosomal DNA sequencing of fecal samples was used to detect the gut microbiota of all subjects. QPCR was used to further verify the species with differences between the two groups. Results The alpha diversity of gut microbiota decreased in CSD patients, accompanied by significant changes of the structure of gut microbiota. Subdoligranulum and Ruminococcus bromii decreased significantly in CSD patients and had a potential diagnostic value for CSD according to receiver operating characteristic curve (ROC) analysis. Enterobacteriaceae and Klebsiella were found to be positively correlated with the duration of CSD, while Clostridium disporicum was positively correlated with the dermatology life quality index (DLQI). Conclusions The gut microbiota of CSD patients is imbalanced. Subdoligranulum and Ruminococcus bromii are the gut microbiota biomarkers in CSD.