Generation of RNAi libraries for high-throughput screens.
Generation of RNAi libraries for high-throughput screens.
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用于高通量屏幕的RNAi库生成。
DOI：
10.1155/jbb/2006/45716
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发表时间：
2006
影响因子：
--
通讯作者：
Ding S
中科院分区：
文献类型：
--
作者：
Clark J;Ding S
多种生物体基因组测序的完成对能够系统分析不断增长的数据的基因组工具产生了巨大的需求。与创建特定敲除细胞系或动物的经典反向遗传学方法耗时且昂贵相比，RNA介导的干扰（RNAi）已成为一种快速、简单且经济有效的大规模基因敲除技术。自从发现 RNAi 技术是对与秀丽隐杆线虫 (C elegans) 内源基因同源的双链 RNA (dsRNA) 的基因沉默反应以来，RNAi 技术已适用于各种高通量筛选 (HTS)，用于全基因组功能丧失 (LOF) 分析。对 RNAi 内源机制的生化见解导致了 RNAi 方法的进步，包括 RNAi 分子合成、递送和序列设计。在本文中，我们将简要回顾这些不同的 RNAi 文库设计，并讨论每种文库策略的优点和缺点。
The completion of the genome sequencing for several organisms has created a great demand for genomic tools that can systematically analyze the growing wealth of data. In contrast to the classical reverse genetics approach of creating specific knockout cell lines or animals that is time-consuming and expensive, RNA-mediated interference (RNAi) has emerged as a fast, simple, and cost-effective technique for gene knockdown in large scale. Since its discovery as a gene silencing response to double-stranded RNA (dsRNA) with homology to endogenous genes in Caenorhabditis elegans (C elegans), RNAi technology has been adapted to various high-throughput screens (HTS) for genome-wide loss-of-function (LOF) analysis. Biochemical insights into the endogenous mechanism of RNAi have led to advances in RNAi methodology including RNAi molecule synthesis, delivery, and sequence design. In this article, we will briefly review these various RNAi library designs and discuss the benefits and drawbacks of each library strategy.