FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media
FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media
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DOI：
10.1016/s1381-1177(99)00030-2
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发表时间：
1999-09-15
影响因子：
--
通讯作者：
Severcan, F
中科院分区：
文献类型：
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作者：
Haris, PI;Severcan, F
随着蛋白质在从药物到生物传感器和生物材料的许多不同应用中的使用越来越多，出现了对不同环境中蛋白质结构表征的需求。在许多情况下，仅仅拥有水中或结晶状态的蛋白质的三维结构是不够的。在有机溶剂、洗涤剂胶束、磷脂膜等存在的情况下，通常需要有关蛋白质结构特性的信息。傅里叶变换红外光谱 (FTIR) 已被确定为少数可用于此类环境中蛋白质结构表征的技术之一。在这里，我们讨论如何使用该技术来获取有关水性和非水性介质中蛋白质结构和稳定性的信息。例子来自我们对水溶性蛋白质和膜蛋白的研究。 (C) 1999 Elsevier Science B.V. 保留所有权利。
With increasing use of proteins in many different applications, ranging from phramaceuticals to biosensors and biomaterials, there has emerged a need for protein structural characterisation in diverse environments. In many cases it is not sufficient to just have the three-dimensional structure of a protein in H2O or in the crystalline state. Often information on the structural properties of a protein is required in the presence of organic solvents, detergent micelles, phospholipid membranes and so on. Fourier transform infrared spectroscopy (FTIR) has been identified as one of the few techniques that can be applied for structural characterisation of proteins in such environments. Here we discuss how this technique is being used to obtain information on protein structure and stability in both aqueous and non-aqueous media. Examples are drawn from our studies of water soluble proteins and membrane proteins. (C) 1999 Elsevier Science B.V. All rights reserved.