The CAMSAP3-ACF7 Complex Couples Noncentrosomal Microtubules with Actin Filaments to Coordinate Their Dynamics
The CAMSAP3-ACF7 Complex Couples Noncentrosomal Microtubules with Actin Filaments to Coordinate Their Dynamics
复制标题
CAMSAP3-ACF7 复合物将非中心体微管与肌动蛋白丝耦合以协调其动力学
DOI：
10.1016/j.devcel.2016.09.003
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发表时间：
2016-10-10
影响因子：
11.8
通讯作者：
Meng, Wenxiang
中科院分区：
文献类型：
--
作者：
Ning, Wenxiu;Yu, Yanan;Meng, Wenxiang
为了适应复杂的细胞功能，需要动态的细胞骨架网络。细胞骨架有两个主要组成部分，微管和肌动蛋白细丝，它们形成了一个复杂的网络，维持着一种精细的合作，为它们的细胞功能建立物理基础。然而，人们对它们协同作用背后的分子机制知之甚少。在这里，我们发现在Caco 2上皮细胞中，非中心体微管通过它们的负端与F-肌动蛋白串扰，并有助于调节焦点黏附大小和细胞迁移。我们发现ACF7是细胞骨架交联蛋白家族中的一员，它与哪吒(又称CAMSAP3)在非中心体微管的负端相互作用，并将它们锚定在肌动蛋白细丝上。这些非中心体微管通过逆行流动与肌动蛋白细丝合作，使其长度和方向垂直于细胞边缘，并调节焦点黏附大小和细胞迁移。
For adaptation to complex cellular functions, dynamic cytoskeletal networks are required. There are two major components of the cytoskeleton, microtubules and actin filaments, which form an intricate network maintaining an exquisite cooperation to build the physical basis for their cellular function. However, little is known about the molecular mechanism underlying their synergism. Here, we show that in Caco2 epithelial cells, noncentrosomal microtubules crosstalk with F-actin through their minus ends and contribute to the regulation of focal adhesion size and cell migration. We demonstrate that ACF7, a member of the spectraplakin family of cytoskeletal crosslinking proteins, interacts with Nezha (also called CAMSAP3) at the minus ends of noncentrosomal microtubules and anchors them to actin filaments. Those noncentrosomal microtubules cooperate with actin filaments through retrograde flow to keep their length and orientation perpendicular to the cell edge as well as regulate focal adhesion size and cell migration.