The BPtpA protein from Burkholderia cenocepacia belongs to a new subclass of low molecular weight protein tyrosine phosphatases.
The BPtpA protein from Burkholderia cenocepacia belongs to a new subclass of low molecular weight protein tyrosine phosphatases.
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来自新洋葱伯克霍尔德杆菌的 BPtpA 蛋白属于低分子量蛋白酪氨酸磷酸酶的新亚类。
DOI：
10.1016/j.abb.2020.108277
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发表时间：
2020
影响因子：
3.9
通讯作者：
Sánchez-Rodríguez R
中科院分区：
文献类型：
--
作者：
Sánchez-Rodríguez R
Low molecular weight protein tyrosine phosphatases (LMW-PTP) are ubiquitous enzymes found across a spectrum of genera from prokaryotes to higher eukaryotes. LMW-PTP belong to the Cys-based PTP class II protein family. Here, we show that LMW-PTP can be categorized into two different groups, referred as class II subdivision I (class II.I) and subdivision II (class II.II). Using BPtpA from the opportunistic pathogenBurkholderia cenocepacia, as a representative member of the LMW-PTP class II.I, we demonstrated that four conserved residues (W47, H48, D80, and F81) are required for enzyme function. Guided by anin silicomodel of BPtpA, we show that the conserved residues at α3-helix (D80 and F81) contribute to protein stability, while the other conserved residues in the W-loop (W47 and H48) likely play a role in substrate recognition. Overall, our results provide new information on LMW-PTP protein family and establishB. cenocepaciaas a suitable model to investigate how substrates are recognized and sorted by these proteins.
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编码非功能性酪氨酸磷酸酶的新洋葱伯克霍尔德杆菌基因是巨噬细胞中含细菌液泡延迟成熟所必需的。
影响因子：
1.5
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.9
作者：
Tabernero,L;Evans,BN;Tishmack,PA;VanEtten,RL;Stauffacher,CV
通讯作者：
Stauffacher,CV
影响因子：
18.3
作者：
Zhang ZY
通讯作者：
Zhang ZY
对酵母 Ltp1 活性位点袋内的两个芳香族残基进行定点诱变。
DOI：
10.1016/j.bbagen.2006.12.012
发表时间：
2007
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
P. Paoli;A. Modesti;F. Magherini;T. Gamberi;Anna Caselli;G. Manao;G. Raugei;G. Camici;G. Ramponi
通讯作者：
G. Ramponi