Use of synthetic peptides as tracer antigens in fluorescence polarization immunoassays of high molecular weight analytes.
Use of synthetic peptides as tracer antigens in fluorescence polarization immunoassays of high molecular weight analytes.
复制标题
在高分子量分析物的荧光偏振免疫测定中使用合成肽作为示踪抗原。
DOI：
10.1021/ac00071a007
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发表时间：
1993
影响因子：
7.4
通讯作者：
Herron,JN
中科院分区：
文献类型：
--
作者：
Wei,AP;Herron,JN
M-1，6 ℃。由于激发态寿命的延长，其荧光强度在结合后增强约20%。在一个典型的实施方案中，hCG测定水平为1 × 10 - 9 M（95%置信限）-比文献中报道的类似系统提高了100倍。这主要归因于示踪剂和抗体之间流体动力学体积的巨大差异，这导致肽示踪剂在结合时的极化的巨大变化。还研究了与敏感性、特异性和可逆性相关的问题。该方法被认为对于快速和经济地测量临床感兴趣的高分子量抗原具有重要意义。
M-1 at 6 C. Its fluorescence intensity was en-hanced by~ 20% upon binding as a result of a prolonged excited-state lifetime. In a typical embodiment, hCG was determined at a level of 1 X 10" 9 M (95% confidence limit)—a 100-fold im-provement over similar systems reported in the literature. This is mainly attributed to the large difference in hydrodynamic volume between the tracer and the antibody, which resulted in large changes in polarization of the peptide tracer upon binding. Issues related to sensitivity, specificity, and reversibility were also investigated. This method is believed to be of significant importance to rapid and economical measurements of high molecular weight antigens of clinical interest.