Isolation and analysis of discreet human prostate cellular populations.
Isolation and analysis of discreet human prostate cellular populations.
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谨慎的人类前列腺细胞群的分离和分析。
DOI：
10.1016/j.diff.2015.10.013
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发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Hayward SW
中科院分区：
文献类型：
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作者：
Strand DW;Aaron L;Henry G;Franco OE;Hayward SW
在转基因小鼠模型中使用谱系追踪揭示了丰富的亚细胞表型，负责维持前列腺稳态。由于组织处理、流式细胞术和细胞培养技术的进步，使用新鲜人体组织来检验这些小鼠实验产生的假设的能力大大增强了。我们详细描述了这些领域的方案优化，以促进通过人体组织分析解决人类前列腺疾病的研究。
The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.