Identification of an Ultrathin Osteochondral Interface Tissue with Specific Nanostructure at the Human Knee Joint
Identification of an Ultrathin Osteochondral Interface Tissue with Specific Nanostructure at the Human Knee Joint
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DOI：
10.1021/acs.nanolett.1c04649
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发表时间：
2022-03-23
期刊：
影响因子：
10.8
通讯作者：
Ouyang, Hongwei
中科院分区：
文献类型：
--
作者：
Wang, Xiaozhao;Lin, Junxin;Ouyang, Hongwei
软骨通过特定的骨软骨界面组织附着在软骨下骨上，在这种界面组织中，力从软软骨转移到硬骨，而不会在整个负载周期中产生疲劳损伤。然而，骨软骨界面组织的精细结构和力学性能尚不清楚。在这里，我们在人类膝关节中发现了一个类似于20-30 μ m梯度钙化区域的超薄层，具有两层微纳米结构的骨软骨界面组织，其具有独特的生物分子组成和复杂的纳米晶体组装。有限元模拟结果表明，在该区域内，模量呈指数增长（3个数量级）有利于力的传递。羟基磷灰石的纳米级非均质性，加上通常存在于肌肉中的弹性反应蛋白titin的富集，赋予骨软骨组织优异的力学性能。总的来说，这些结果为骨软骨界面再生的高性能界面材料的潜在设计提供了新的见解。
Cartilage adheres to subchondral bone via a specific osteochondral interface tissue where forces are transferred from soft cartilage to hard bone without conferring fatigue damage over a lifetime of load cycles. However, the fine structure and mechanical properties of the osteochondral interface tissue remain unclear. Here, we identified an ultrathin similar to 20-30 mu m graded calcified region with two-layered micronano structures of osteochondral interface tissue in the human knee joint, which exhibited characteristic biomolecular compositions and complex nanocrystals assembly. Results from finite element simulations revealed that within this region, an exponential increase of modulus (3 orders of magnitude) was conducive to force transmission. Nanoscale heterogeneity in the hydroxyapatite, coupled with enrichment of elastic-responsive protein-titin, which is usually present in muscle, endowed the osteochondral tissue with excellent mechanical properties. Collectively, these results provide novel insights into the potential design for high-performance interface materials for osteochondral interface regeneration.