Electroporation-Mediated Immunization of a Candidate DNA Vaccine Expressing Dengue Virus Serotype 4 prM-E Antigen Confers Long-Term Protection in Mice
Electroporation-Mediated Immunization of a Candidate DNA Vaccine Expressing Dengue Virus Serotype 4 prM-E Antigen Confers Long-Term Protection in Mice
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表达登革热病毒血清型 4 prM-E 抗原的候选 DNA 疫苗的电穿孔介导免疫可为小鼠提供长期保护
DOI：
10.1007/s12250-019-00090-8
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发表时间：
2019-02
影响因子：
5.5
通讯作者：
Jing An
中科院分区：
文献类型：
--
作者：
Ziyang Sheng;Hui Chen;Kaihao Feng;Na Gao;Ran Wang;Peigang Wang;Dongying Fan;Jing An
登革热由登革热病毒(DENV)引起，是一种广泛传播的蚊媒人畜共患病；然而，目前还没有可供全球使用的抗登革热疫苗。本研究构建了表达DENV 4型(DENV-4)PrM-E蛋白的DNA疫苗候选株(PV-D4ME)，并对其免疫原性和免疫保护性进行了研究。PV-D4ME候选疫苗在50μg和5μg电穿孔接种时，均能诱导小鼠有效的体液免疫和细胞免疫。在接受三次免疫接种两周后，两种剂量的PV-D4ME DNA被证明对致命的DENV-4攻击具有有效的保护作用。值得注意的是，在三次免疫后6个月，50μg而不是5μg的PV-D4ME可以对DENV-4致死攻击提供稳定的保护(100%存活率)，而没有任何明显的临床症状。这些结果表明，用50μg PV-D4ME电穿孔免疫小鼠可获得长期有效的免疫保护，为新型疫苗的研制提供了一条新的途径。
Dengue fever, caused by dengue viruses (DENVs), is a widespread mosquito-borne zoonotic disease; however, there is no available anti-dengue vaccine for worldwide use. In the current study, a DNA vaccine candidate (pV-D4ME) expressing prM-E protein of DENV serotype 4 (DENV-4) was constructed, and its immunogenicity and protection were evaluated in immunocompetent BALB/c mice. The pV-D4ME candidate vaccine induced effective humoral and cellular immunity of mice against DENV-4in vivowhen administered both at 50 μg and 5 μg through electroporation. Two weeks after receiving three immunizations, both doses of pV-D4ME DNA were shown to confer effective protection against lethal DENV-4 challenge. Notably, at 6 months after the three immunizations, 50 μg, but not 5 μg, of pV-D4ME could provide stable protection (100% survival rate) against DENV-4 lethal challenge without any obvious clinical signs. These results suggest that immunization with 50 μg pV-D4ME through electroporation could confer effective and long-term protection against DENV-4, offering a promising approach for development of a novel DNA vaccine against DENVs.
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