USE OF AMINO-ACIDS BY PLASMODIUM-RELICTUM OOCYSTS INVITRO
USE OF AMINO-ACIDS BY PLASMODIUM-RELICTUM OOCYSTS INVITRO
复制标题
DOI：
10.1016/0014-4894(76)90018-7
复制
发表时间：
1976-01-01
影响因子：
2.1
通讯作者：
CHAO, J
中科院分区：
文献类型：
--
作者：
BALL, GH;CHAO, J
本文比较了在添加胎牛血清的Graces昆虫培养基中，在存在天蚕分裂细胞的情况下，跗库蚊肠道的体外生长情况，与用鸟类寄生虫P. relictum的卵囊感染的肠道的类似制备情况。在后一种情况下，16小时后，精氨酸、天冬酰胺和谷氨酰胺组合、谷氨酸、甘氨酸、组氨酸、赖氨酸、脯氨酸和丝氨酸的浓度发生显著降低。丙氨酸、β-丙氨酸和β-丙氨酸浓度的降低更低和更不显著。发生丙氨酸、胱氨酸、异亮氨酸、亮氨酸、甲硫氨酸、鸟氨酸、苯丙氨酸、苏氨酸、色氨酸、酪氨酸和缬氨酸。这表明在代谢昆虫细胞的存在下，残遗拟单胞菌的发育卵囊利用某些氨基酸。
A comparison was made of the in vitro growth of the gut of Culex tarsalis in Grace''s insect culture medium, supplemented with fetal bovine serum in the presence of dividing cells of Antheraea eucalypti, with a similar preparation of a gut infected with oocysts of the avian parasite P. relictum. In the latter case, after 16 h significant decreases occurred in the concentration of arginine, asparagine and glutamine combined, glutamic acid, glycine, histidine, lysine, proline and serine. Lower and less marked decreased concentrations of alanine, .beta.-alanine, cystine, isoleucine, leucine, methionine, ornithine, phenylalanine, threonine, tryptophan, tyrosine and valine took place. This indicated utilization of certain amino acids by the developing oocysts of P. relictum in the presence of metabolizing insect cells.