Osteoblast-like cell response to macro- and micro-patterned carbon scaffolds obtained from the sea rush Juncus maritimus
Osteoblast-like cell response to macro- and micro-patterned carbon scaffolds obtained from the sea rush Juncus maritimus
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DOI：
10.1088/1748-6041/6/4/045012
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发表时间：
2011-08
影响因子：
4
通讯作者：
M. López‐Álvarez;I. Pereiro;J. Serra;A. de Carlos;P. González
中科院分区：
文献类型：
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作者：
M. López‐Álvarez;I. Pereiro;J. Serra;A. de Carlos;P. González
通过热解海草Juncus maritimus制备了具有定向花纹表面的碳支架。用扫描电子显微镜、压汞测孔仪和干涉轮廓术对支架的结构进行了表征。用X射线衍射法和X射线荧光法对其进行了化学表征。通过扫描电子显微镜、激光共聚焦扫描显微镜、磷酸酶碱性活性和骨钙素合成的定量检测，观察成骨细胞(MC3T3-E1细胞系)在图案化支架上的排列和分化情况。研究发现，500℃下的热解保留甚至增强了植物的自然宏观和微观图案。孔隙率和化学组成的结果验证了这些结构是组织工程应用的可行支架。最后，图案化的表面不仅在短期(几小时)培养后，而且在较长时间(数周)培养后，证实了该表面促进了成骨前细胞MC3T3-E1的定向生长。细胞分化标志物的定量和培养28d时细胞层形态的评价证实了MC3T3-E1细胞向成骨细胞分化。
Carbon scaffolds with a directional patterned surface were obtained by pyrolysis of the sea rush Juncus maritimus. The structure of the scaffolds was investigated using scanning electron microscopy, mercury porosimetry and interferometric profilometry. X-ray diffraction and x-ray fluorescence were the techniques used for their chemical characterization. The alignment and differentiation of pre-osteoblasts (MC3T3-E1 cell line) incubated on the patterned scaffolds were evaluated by scanning electron microscopy, confocal laser scanning microscopy and by the quantification of the phosphatase alkaline activity and the osteocalcin synthesis. It was found that pyrolysis at 500 °C preserved and even enhanced the natural macro- and micro-patterning of the plant. The results obtained for porosity and chemical composition validated these structures as viable scaffolds for tissue engineering applications. Finally, the patterned surface was confirmed to promote the oriented growth of the pre-osteoblasts MC3T3-E1, not only after short periods of incubation (hours) but also after longer ones (several weeks). The quantification of the cell differentiation markers together with the evaluation of the cell layer morphology up to 28 days of incubation confirmed the differentiation of MC3T3-E1 cells to osteoblasts.