Morphotypic and molecular identification of filamentous fungi from Spanish blue-veined Cabrales cheese, and typing of Penicillium roqueforti and Geotrichum candidum isolates
Morphotypic and molecular identification of filamentous fungi from Spanish blue-veined Cabrales cheese, and typing of Penicillium roqueforti and Geotrichum candidum isolates
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DOI：
10.1016/j.idairyj.2006.04.002
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发表时间：
2007-04-01
影响因子：
3.1
通讯作者：
Mayo, Baltasar
中科院分区：
文献类型：
--
作者：
Belen Florez, Ana;Alvarez-Martin, Pablo;Mayo, Baltasar
三十五白色和蓝绿色丝状真菌，占主导地位的Cabrales奶酪成熟过程中，形态和基因型鉴定。形态分析鉴定为青霉属。(12分离株）、罗克福尔青霉（11个分离株）、白地霉（10个分离株）、夏蒂生枝顶孢菌（1个分离株）以及1个无法鉴定的分离株。PCR扩增产物经6种限制性内切酶进行限制性片段长度多态性（RFLP）分析。形态上鉴定为P. roqueforti的菌株都显示出与所有酶相同的RFLP图谱，所有G. candidum菌株;确认其表型分类。分子生物学分析表明，青霉属（Penicillium spp.）群由普通青霉（5个分离物）、产黄青霉（4个分离物）、洛克福特青霉（2个分离物）和柠檬黑青霉（1个分离物）组成。不同种间的遗传多样性分析表明，用两对引物进行快速扩增DNA聚合酶链反应（RAPD-PCR），对10株念珠菌进行鉴定。在13个分离株中鉴定出9株罗克福地单胞菌，每株罗克福地单胞菌均为G. Candidum分离物代表单个菌株。(c)2006爱思唯尔有限公司保留所有权利。
Thirty five white and blue-greenish filamentous fungi, dominant during ripening of Cabrales cheese, were morphotypically and genotypically identified. Morphotypic analysis identified Penicillium spp. (12 isolates), Penicillium roqueforti (11 isolates), Geotrichum candidum (10 isolates), Acremonium charticola (1 isolate), and one isolate that could not be identified. Polymerase chain reaction (PCR) amplicons of their ITS1-5.8S-ITS2 region were subjected to Restriction fragment length polymorphism (RFLP) analysis with six restriction enzymes. Strains morphotypically identified as P. roqueforti all showed identical RFLP profiles with all enzymes, as did all the G. candidum strains; confirming their phenotypic classification. However, molecular analysis showed the Penicillium spp. group to be composed of P. commune (5 isolates), P. chrysogenum (4 isolates), P. roqueforti (2 isolates), and P. citreonigrum (1 isolate). The genetic diversity found among the different P. roqueforti and G. candidum isolates was assessed by Rapid amplified polymeric DNA-polymerase chain reaction (RAPD-PCR) with two primers. Nine P. roqueforti strains were defined among the 13 isolates, and every G. candidum isolate represented an individual strain. (c) 2006 Elsevier Ltd. All rights reserved.