Cytometric bead array: a multiplexed assay platform with applications in various areas of biology
Cytometric bead array: a multiplexed assay platform with applications in various areas of biology
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DOI：
10.1016/j.clim.2003.11.017
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发表时间：
2004-03-01
影响因子：
8.6
通讯作者：
Gaur, A
中科院分区：
文献类型：
--
作者：
Morgan, E;Varro, R;Gaur, A
基于流式细胞仪微珠技术的引入为研究人员同时测量生物和环境样品中的多种分析物增加了一种新方法。这种新技术允许（1）在单个样品中评估多种分析物;（2）利用最小的样品体积收集数据;（3）与先前实验的再现性和结果比较;（4）与现有测定直接比较;以及（5）在单个平台中更快速地评估多个样品。流式细胞仪微珠阵列（CBA）系统能够同时测量样品体积太小，传统的免疫测定的多个分析物。结果已被提交的各种人类细胞因子的分析。此外，该技术允许设计和创建测定以测量各种分析物，包括炎症介质、趋化因子、免疫球蛋白同种型、细胞内信号传导分子、凋亡介质、粘附分子和抗体。人类基因组计划和FDA提出的新倡议要求开发和使用快速同时定量多种分析物的测定法。CBA技术提供了定量给定样品中多种蛋白质的能力，具有精确性和一致性。(C)2004年爱思唯尔公司All rights reserved.
The introduction of flow cytometric bead-based technology has added a new approach for investigators to simultaneously measure multiple analytes in biological and environmental samples. This new technology allows for (1) evaluation of multiple analytes in a single sample; (2) utilization of minimal sample volumes to glean data; (3) reproducibility and results comparative with previous experiments; (4) direct comparison with existing assays; and (5) a more rapid evaluation of multiple samples in a single platform. The cytometric bead array (CBA) system enables simultaneous measurement of multiple analytes in sample volumes too small for traditional immunoassays. Results have been presented for the analysis of a variety of human cytokines. In addition, the technology allows for the design and creation of assays to measure a variety of analytes including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies. New initiatives put forward by the Human Genome Project and the FDA require the development and use of assays for the rapid simultaneous quantitation of multiple analytes. The CBA technology provides the ability to quantify multiple proteins within a given sample, with precision and consistency. (C) 2004 Elsevier Inc. All rights reserved.