A juvenile murine heart failure model of pressure overload.
A juvenile murine heart failure model of pressure overload.
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压力超负荷的幼年小鼠心力衰竭模型。
DOI：
10.1007/s00246-010-9833-3
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发表时间：
2011
影响因子：
1.6
通讯作者：
Wang,Yanggan
中科院分区：
文献类型：
--
作者：
Cumbermack,KristopherM;Cheng,Jun;Nong,Yibing;Mahle,WilliamT;Joyner,RonaldW;Border,WilliamL;Wagner,MaryB;Fyfe,DerekA;Leong,Traci;Wang,Yanggan
持续的压力超负荷可导致心脏肥大和进行性心力衰竭（HF）。作者开发了幼年小鼠压力过载心力衰竭模型，以研究心脏对压力过载的反应，这可能适用于儿童的临床过程。使用 28 号针对 40 只幼年（3 周）和 47 只成年（6 周）C57BL/6 雄性小鼠进行严重胸主动脉结扎 (sTAB)。为了监测结构和功能的变化，对接受 sTAB 和假手术的清醒小鼠进行 M 型超声心动图检查。 sTAB 后幼年和成年小鼠均出现心脏肥大、扩张和心力衰竭。与成人相比，青少年心力衰竭的特点是心室收缩力受损较大，肥厚程度较轻。此外，在术后早期几周内，幼年小鼠的存活率明显高于成年小鼠。与儿童中观察到的临床心力衰竭一致，幼年带状小鼠的生长速度低于成年带状小鼠或进行假手术的幼年对照小鼠。作者首先开发了压力过载心力衰竭的幼年小鼠模型。了解青少年压力超负荷心力衰竭的独特特征应有助于了解儿童心力衰竭发展的年龄特异性病理变化。
Persistent pressure overload can cause cardiac hypertrophy and progressive heart failure (HF). The authors developed a pressure-overload HF model of juvenile mice to study the cardiac response to pressure overload that may be applicable to clinical processes in children. Severe thoracic aortic banding (sTAB) was performed using a 28-gauge needle for 40 juvenile (age, 3 weeks) and 47 adult (age, 6 weeks) C57BL/6 male mice. To monitor the structural and functional changes, M-mode echocardiography was performed for conscious mice that had undergone sTAB and sham operation. Cardiac hypertrophy, dilation, and HF occurred in both juvenile and adult mice after sTAB. Compared with adults, juvenile HF is characterized by greater impairment of ventricular contractility and less hypertrophy. In addition, juvenile mice had significantly higher rates of survival than adult mice during the early postoperative weeks. Consistent with clinical HF seen in children, juvenile banded mice demonstrated a lower growth rate than either adult banded mice or juvenile control mice that had sham operations. The authors first developed a juvenile murine model of pressure-overload HF. Learning the unique characteristics of pressure-overload HF in juveniles should aid in understanding age-specific pathologic changes for HF development in children.