2-Phenylethylamine catabolism by Escherichia coli K12.
2-Phenylethylamine catabolism by Escherichia coli K12.
复制标题
大肠杆菌 K12 的 2-苯乙胺分解代谢。
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
R. Cooper
中科院分区：
文献类型：
--
作者：
S. Parrott;S. Jones;R. Cooper
大肠杆菌K12以2-苯乙胺作为唯一的碳源和能源，通过苯乙醛将其转化为苯乙酸。苯乙醛是通过诱导型胺氧化酶的作用形成的，过氧化氢酶活性增加了六倍，大概是为了确保去除预计是胺氧化产物的H2 O2。苯乙醛被诱导型NAD+依赖性脱氢酶氧化为苯乙酸。苯乙醛脱氢酶缺陷的突变体不能以2-苯乙胺作为碳源和能源生长，但仍可以将其用作氮源。
Escherichia coli K12 grows on 2-phenylethylamine as sole carbon and energy source by converting it, via phenylacetaldehyde, to phenylacetic acid. Phenylacetaldehyde was formed by the action of an inducible amine oxidase and catalase activity was increased sixfold, presumably to ensure removal of the H2O2 that was expected to be a product of the amine oxidation. The phenylacetaldehyde was oxidized to phenylacetic acid by an inducible NAD+-dependent dehydrogenase. Mutants defective in phenylacetaldehyde dehydrogenase cannot grow on 2-phenylethylamine as carbon and energy source but can still use it as a nitrogen source.