Mining the Watkins collection of wheat landraces for novel sources of eyespot resistance
Mining the Watkins collection of wheat landraces for novel sources of eyespot resistance
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DOI：
10.1111/ppa.12221
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发表时间：
2014-12-01
期刊：
影响因子：
2.7
通讯作者：
Nicholson, P.
中科院分区：
文献类型：
--
作者：
Burt, C.;Griffe, L. L.;Nicholson, P.
小麦褐斑病是由土传病原菌引起的小麦茎基部病害。控制该病最有效的方法是宿主抗性。然而，在小麦品种中只有三种遗传特征抗性，需要进一步的抗性来源。以前的研究已经确定了野生近缘种的抗性，但这些抗性的使用受到连锁阻力的限制，这些阻力具有因重组率低而加剧的有害性状。因此，在六倍体小麦种质中鉴定新的抗性是必要的。沃特金斯收集目前包括1056个六倍体小麦地方品种，代表了20世纪20年代和30年代收集时的全球小麦多样性。因此，它可能含有有益的农艺性状，如抗眼斑性。根据对所有1056份材料的温室试验和对从先前田间试验中选择的44份材料的多通道试验，筛选Watkins收集物对O.yallundae的抗性。在这些试验中鉴定的抗性品系对Yallundae和O. acuformis进行了重新试验。这确定了17个加入一个或两个病原体物种的抗性。从这些，两个加入（1190094.1和1190736.3）提供了一个高水平的抵抗这两种病原体物种。来源于登记号1190736.3的F-4群体表明，该登记号中对尖形稻瘟病菌的抗性由单个基因赋予，因此适合于渐渗到优良小麦品种中以提供眼斑病抗性的替代来源。
Eyespot is an economically important stem base disease of wheat caused by the soilborne fungal pathogens Oculimacula yallundae and Oculimacula acuformis. The most effective method of controlling the disease is host resistance. However, there are only three genetically characterized resistances in wheat varieties and further sources of resistance are required. Previous studies have identified resistances in wild relatives, but use of these resistances has been limited by linkage drag with deleterious traits exacerbated by low rates of recombination. Therefore, the identification of novel resistances in hexaploid wheat germplasm is desirable. The Watkins collection currently consists of 1056 hexaploid wheat landraces that represent global wheat diversity at the time of its collection in the 1920s and 1930s. As such, it may contain beneficial agronomic traits such as eyespot resistance. The Watkins collection was screened for resistance to O.yallundae based on a glasshouse test of all 1056 accessions and a polytunnel test of 44 accessions selected from a previous field trial. Resistant lines identified in these tests were retested against both O.yallundae and O.acuformis. This identified 17 accessions with resistance to one or both of the pathogen species. From these, two accessions (1190094.1 and 1190736.3) provided a high level of resistance to both pathogen species. An F-4 population derived from accession 1190736.3 indicated that the resistance to O.acuformis in this accession is conferred by a single gene and therefore would be suitable for introgression into elite wheat varieties to provide an alternative source of eyespot resistance.