First Microsatellites From Spodoptera frugiperda (Lepidoptera: Noctuidae) and Their Potential Use for Population Genetics
First Microsatellites From Spodoptera frugiperda (Lepidoptera: Noctuidae) and Their Potential Use for Population Genetics
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DOI：
10.1603/an10135
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发表时间：
2011-05-01
影响因子：
2.3
通讯作者：
Scheffler, Brian E.
中科院分区：
文献类型：
--
作者：
Arias, Renee S.;Blanco, Carlos A.;Scheffler, Brian E.
本文首次报道了草地贪夜蛾（Spodoptera frugiperda（J.E.）史密斯）（鳞翅目：夜蛾科），美洲重要的经济作物害虫。我们通过焦磷酸测序分离出192个微卫星标记，并从三个地理区域：波多黎各（PR），德克萨斯州（TX）和密西西比（MS）收集的8个同系中筛选了15个个体。还包括对来自苏云金芽孢杆菌（Berliner）（芽孢杆菌目：芽孢杆菌科）的Cry毒素具有抗性的同科。进行聚类分析，以确定这些SSR在区分人群中的潜在用途，并将菌落分组，通过自助法估计可靠性为100%。在该分析中，来自TX的菌落远离来自PR的菌落分组，但两个MS同系分别与TX和PR分组。同系内的遗传距离在0.22 ~ 0.56之间，同系间的最小遗传距离为0.83。计算独特模式信息组合（UPIC）评分，并根据其区分潜力列出UPIC评分>= 1的80个SSR标记。UPIC评分允许通过选择更少且信息量高的标记物用于未来研究来降低成本。从最好的125个标记中，103个每个样本最多有两个等位基因，使它们成为群体遗传研究的理想候选者。序列的BLAST筛选指向标记多态性的潜在生物学意义。三个地理区域共有的等位基因百分比为14%。所报道的标记将显著丰富S.食虫类。此外，它们还可用于监测种群的迁移、开发生物控制剂和一般的管理做法。
This is the first report of sequence-specific microsatellite markers (simple sequence repeats [SSRs]) of fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), an economically important pest of crops on the Americas. We isolated 192 microsatellite markers by using pyrosequencing and screened 15 individuals from eight isofamilies collected from three geographical areas: Puerto Rico (PR), Texas (TX), and Mississippi (MS). Isofamilies resistant to Cry toxins from Bacillus thuringiensis (Berliner) (Bacillales: Bacillaceae) also were included. Cluster analysis was performed to determine the potential use of these SSRs in discriminating populations, and colonies were grouped with a reliability of 100% estimated by bootstrap. In this analysis, colonies from TX grouped away from those from PR, but the two MS isofamilies grouped with TX and PR separately. Genetic distance within isofamilies ranged between 0.22 and 0.56, and the minimum distance between isofamilies was 0.83. Unique pattern informative combination (UPIC) scores were calculated, and the 80 SSR markers that had UPIC scores of >= 1 are listed according to their discriminating potential. UPIC scores allow reducing costs by choosing fewer and highly informative markers for future studies. From the best 125 markers, 103 had a maximum of two alleles per sample, making them ideal candidates for population genetic studies. BLAST screening of the sequences points to potential biological meaning of marker polymorphisms. The percentage of alleles shared by the three geographic areas was 14%. The markers reported will significantly enrich the pool of molecular markers available for S. frugiperda. In addition, they could be used for monitoring migration of populations, in the development of biocontrol agents and for management practices in general.