Salivary microRNAs and oral cancer detection.
Salivary microRNAs and oral cancer detection.
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DOI：
10.1007/978-1-62703-083-0_24
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发表时间：
2013
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人类唾液中的微小RNA（miRNAs）近年来已成为唾液研究中的一个新兴领域，用于诊断应用及其在生物学意义上的潜在作用。miRNAs是一种短的非编码RNA分子，在调节多种细胞过程中发挥重要作用。已知miRNA的失调与许多疾病相关。miRNAs存在于口腔鳞癌患者的唾液中，可作为口腔癌检测的潜在生物标志物。了解miRNAs与疾病相关的生物学功能对于利用miRNAs作为诊断标志物非常重要。目前有多种分析方法可用于检测miRNA表达水平。在本章中，我们概述了应用生物系统干环RT的Taqman MicroRNA检测唾液miRNA谱。使用这种高度敏感和特异性的测定，唾液中的miRNA仅用几纳克的起始RNA进行分析。该方法也适用于研究含有少量RNA的其他体液或临床样品中的生物标志物。
MicroRNAs (miRNAs) in human saliva have recently become an emerging field in saliva research for diagnostics applications and its potential role in biological implications. miRNAs are short noncoding RNA molecules that play important roles in regulating a variety of cellular processes. Dysregulation of miRNAs are known to be associated with many diseases. miRNAs were found present in the saliva of OSCC patients and could serve as potential biomarkers for oral cancer detection. Understanding the biological function of miRNAs in association with diseases is important towards utilizing miRNAs as diagnostic markers. There are currently a variety of profiling methods available for detecting miRNA expression levels. In this chapter, we overview the Applied Biosystem Stem-loop RT based Taqman MicroRNA Assay for salivary miRNA profiling. Using this highly sensitive and specific assay, miRNAs in saliva are profiled with only a few nanograms of starting RNA. This method is also applicable for studying biomarkers in other body fluids or clinical samples that contain small amounts of RNA.