Meiotic anomalies in infertile men with severe spermatogenic defects
Meiotic anomalies in infertile men with severe spermatogenic defects
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DOI：
10.1093/humrep/deh868
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发表时间：
2005-07-01
影响因子：
6.1
通讯作者：
Grillo, JM
中科院分区：
文献类型：
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作者：
Guichaoua, MR;Perrin, J;Grillo, JM
背景：本研究旨在评估梗阻性(O)和非梗阻性(NO)不育患者粗线期精母细胞配对失败率。方法：41名患者和13名对照组接受了睾丸活检。O型不孕症19例，非不孕症22例。所有患者和对照组的标本进行Giemsa染色，其中9例患者和1名对照组的联会复合体进行免疫组织化学染色。结果：总共分析了2931个粗线核。NO组神经核平均突触率(25.4%)明显高于O组(9.8%)。O组与对照组比较差异无统计学意义(10.6%)。免疫细胞化学结果显示，从粗线期早期到晚期，所有患者的粗线期细胞核数量均减少。2例NO患者，1例无精子症患者和1例少精子症患者，有较高比例的非突触核(分别为86%和91.8%)；其中1例患者还表现出姐妹染色单体的早熟局限性分离。结论：高水平的广泛性非突触可能源于初级减数分裂缺陷，这可能是本中心9%的无男性不育的原因。早期粗线期的流行表明粗线期检查点位于人类粗线期中期。
BACKGROUND: This study was aimed at evaluating the rate of pairing failure in pachytene spermatocytes of patients presenting either an obstructive (O) or a non-obstructive (NO) infertility.METHODS: Forty-one patients and 13 controls underwent testicular biopsy. Among the patients, 19 had an O infertility and 22 a NO infertility. Preparations of all patients and controls were Giemsa-stained, and synaptonemal complexes from nine of these patients and one control were immunostained.RESULTS: In all, 2931 pachytene nuclei were analysed. The mean rate of asynapsed nuclei from the NO group (25.4%) was significantly higher than that of the O group (9.8%). There was no significant difference between the O group and the controls (10.6%). Immunocytochemistry showed that the number of pachytene nuclei decreased from the early to late pachytene sub-stage in all patients. Two NO patients, one azoospermic and one oligozoospermic, had a high percentage of asynapsed nuclei (86 and 91.8% respectively); one of these patients also presented a precocious localized separation of sister chromatids.CONCLUSION: high levels of extended asynapsis could arise from a primary meiotic defect which may be responsible for 9% of the NO male infertilities at our centre. The prevalence of early pachytene substages suggests that the pachytene checkpoint is localized at the mid-pachytene stage in humans.